| 1 | Kaylin Irvine (Senior) | CAN | 38.89 | | - |
| 2 | Shannon Rempel (Senior) | CAN | 39.05 | | +0.160 |
| 3 | Ireen Wüst (Senior) | NED | 39.10 | | +0.210 |
| 4 | Nana Takagi (Neo) | JPN | 39.14 | | +0.250 |
| 5 | Alexandra Ianculescu (Neo) | CAN | 39.14 | | +0.250 |
| 6 | Ivanie Blondin (Senior) | CAN | 39.28 | | +0.390 |
| 7 | Luiza Złotkowska (Senior) | POL | 39.43 | SB | +0.540 |
| 8 | Ida Njåtun (Senior) | NOR | 39.45 | | +0.560 |
| 9 | Linda de Vries (Senior) | NED | 39.47 | | +0.580 |
| 10 | Marije Joling (Senior) | NED | 39.63 | | +0.740 |
| 11 | Tamara Oudenaarden (Senior) | CAN | 39.77 | | +0.880 |
| 12 | Kate Hanly (Neo) | CAN | 40.03 | | +1.140 |
| 13 | Josie Spence (Neo) | CAN | 40.19 | SB | +1.300 |
| 14 | Nikola Zdráhalova (A2) | CZE | 40.25 | SB | +1.360 |
| 15 | Natalia Voronina (Neo) | RUS | 40.26 | | +1.370 |
| 16 | Claudia Pechstein (Senior) | GER | 40.35 | | +1.460 |
| 17 | Keira Poelzer (Neo) | CAN | 40.36 | | +1.470 |
| 18 | Olga Graf (M30) | RUS | 40.45 | | +1.560 |
| 19 | Rikke Jeppsson (Senior) | NOR | 40.51 | | +1.620 |
| 20 | Leia Marie Behlau (A2) | GER | 41.60 | | +2.710 |
| 21 | Victoria Spence (Neo) | CAN | 41.70 | | +2.810 |
| 22 | Nicole Garrido (Senior) | CAN | 41.73 | | +2.840 |
| 23 | Allison Bates (Neo) | CAN | 42.04 | | +3.150 |
| 24 | Caitlyn MacDonald (Neo) | CAN | 43.38 | | +4.490 |
| 25 | Michelle Daeninck (A2) | CAN | 43.59 | | +4.700 |
| 26 | Emilie Medland-Marchen (A2) | CAN | 43.94 | | +5.050 |
| 27 | Danielle Wallish (Neo) | CAN | 43.99 | | +5.100 |
| DNF | Tabea Theurich (B2) | GER | DNF | | - |