| 1 | Erin Jackson (Senior) | USA | 37.31 | SB | - |
| 2 | Brittany Bowe (Senior) | USA | 38.05 | SB | +0.740 |
| 3 | Chrysta Rands-Evans (Senior) | USA | 38.38 | SB | +1.070 |
| 4 | Sarah Warren (Senior) | USA | 38.39 | SB | +1.080 |
| 5 | McKenzie Browne (Senior) | USA | 38.94 | | +1.630 |
| 6 | Kimi Goetz (Senior) | USA | 39.33 | SB | +2.020 |
| 7 | Blair Cruikshank (Senior) | USA | 39.61 | SB | +2.300 |
| 8 | Anna Quinn (Senior) | USA | 40.46 | | +3.150 |
| 9 | Piper Yde (Neo) | USA | 40.77 | | +3.460 |
| 10 | Libby Williams (Neo) | USA | 40.78 | SB | +3.470 |
| 11 | Ilsa Melin Sandvig Shobe (Senior) | USA | 41.24 | PB | +3.930 |
| 12 | Marley Soldan (A2) | USA | 41.26 | | +3.950 |
| 13 | Samantha Scheffler (Senior) | USA | 41.84 | PB | +4.530 |
| 14 | Natasa Gakovic (Neo) | USA | 41.90 | PB | +4.590 |
| 15 | Keeley Whitcomb (A1) | USA | 41.96 | PB | +4.650 |
| 16 | Elise Stave (A1) | USA | 42.38 | SB | +5.070 |
| 17 | Evie Forrer (B2) | USA | 42.97 | PB | +5.660 |
| 18 | Ana KImber (Senior) | USA | 42.98 | PB | +5.670 |
| 19 | Leah Choi (A1) | USA | 43.18 | | +5.870 |
| 20 | Elena Stave (B1) | USA | 43.42 | SB | +6.110 |
| 21 | Cynthia Jennings (Senior) | USA | 43.57 | | +6.260 |
| 22 | Sydney-Yu Terpening (Senior) | USA | 44.41 | | +7.100 |
| 23 | Elliana Fickel (B1) | USA | 45.84 | PB | +8.530 |
| 24 | Juliette Song (C2) | USA | 47.14 | PB | +9.830 |