| 1 | Erin Jackson (Senior) | USA | 37.76 | SB | - |
| 2 | Brittany Bowe (Senior) | USA | 38.20 | | +0.440 |
| 3 | Chrysta Rands-Evans (Senior) | USA | 38.75 | | +0.990 |
| 4 | Sarah Warren (Senior) | USA | 38.80 | SB | +1.040 |
| 5 | McKenzie Browne (Senior) | USA | 39.01 | SB | +1.250 |
| 6 | Kimi Goetz (Senior) | USA | 39.35 | SB | +1.590 |
| 7 | Blair Cruikshank (Senior) | USA | 39.80 | SB | +2.040 |
| 8 | Anna Quinn (Senior) | USA | 40.31 | SB | +2.550 |
| 9 | Libby Williams (Neo) | USA | 41.00 | SB | +3.240 |
| 10 | Marley Soldan (A2) | USA | 41.11 | PB | +3.350 |
| 11 | Ilsa Melin Sandvig Shobe (Senior) | USA | 41.36 | SB | +3.600 |
| 12 | Samantha Scheffler (Senior) | USA | 42.08 | PB | +4.320 |
| 13 | Keeley Whitcomb (A1) | USA | 42.45 | PB | +4.690 |
| 14 | Elise Stave (A1) | USA | 42.75 | SB | +4.990 |
| 15 | Evie Forrer (B2) | USA | 43.13 | PB | +5.370 |
| 16 | Ana KImber (Senior) | USA | 43.31 | PB | +5.550 |
| 17 | Elena Stave (B1) | USA | 44.34 | | +6.580 |
| 18 | Leah Choi (A1) | USA | 44.41 | | +6.650 |
| 19 | Cynthia Jennings (Senior) | USA | 44.42 | | +6.660 |
| 20 | Sydney-Yu Terpening (Senior) | USA | 44.98 | | +7.220 |
| 21 | Natasa Gakovic (Neo) | USA | 45.98 | | +8.220 |
| 22 | Elliana Fickel (B1) | USA | 46.76 | PB | +9.000 |
| 23 | Juliette Song (C2) | USA | 47.69 | SB | +9.930 |
| DQ | Piper Yde (Neo) | USA | DQ | | - |