| 1 | Kimi Goetz (Senior) | USA | 37.52 | SB | - |
| 2 | Erin Jackson (Senior) | USA | 37.59 | | +0.070 |
| 3 | Brianna Bocox (Senior) | USA | 38.65 | | +1.130 |
| 4 | Paige Schwartzburg (Senior) | USA | 39.73 | SB | +2.210 |
| 5 | Mia Kilburg-Manganello (Senior) | USA | 39.80 | | +2.280 |
| 6 | Blair Cruikshank (Neo) | USA | 39.95 | SB | +2.430 |
| 7 | Chrysta Rands (Senior) | USA | 40.43 | SB | +2.910 |
| 8 | Maria Victoria Rodriguez (Senior) | ARG | 41.10 | | +3.580 |
| 9 | Anna Quinn (Neo) | USA | 41.22 | SB | +3.700 |
| 10 | Jamie Jurak (Senior) | USA | 41.46 | PB | +3.940 |
| 11 | Jenni Kukkonen (Senior) | FIN | 41.54 | SB | +4.020 |
| 12 | Laura Michelle Rodriguez (C2) | COL | 42.00 | NRJ | +4.480 |
| 13 | Dessie Weigel (Senior) | USA | 42.02 | | +4.500 |
| 14 | Darian O'Neil (Senior) | USA | 42.05 | | +4.530 |
| 15 | Natalia Valencia (Neo) | COL | 42.50 | | +4.980 |
| 16 | Sydney-Yu Terpening (Neo) | USA | 42.92 | | +5.400 |
| 17 | Giorgia Birkeland (A2) | USA | 42.93 | | +5.410 |
| 18 | Ranja Ezzi (M35) | SWE | 43.11 | NRM | +5.590 |
| 19 | Ilsa Melin Sandvig Shobe (Neo) | USA | 43.42 | | +5.900 |
| 20 | Shruti Kotwal (Senior) | IND | 43.43 | NR | +5.910 |
| 21 | Thalia Staehle (B2) | USA | 43.83 | | +6.310 |
| 22 | Cynthia Jennings (Senior) | USA | 44.26 | SB | +6.740 |
| 23 | Isabela Vargas Rodriguez (C1) | COL | 44.97 | | +7.450 |
| 24 | Ella Teeples (D1) | USA | 48.22 | SB | +10.700 |
| 25 | Mariana Caro (n/a) | COL | 48.47 | PB | +10.950 |
| 26 | Avery Hayes (D1) | USA | 51.63 | | +14.110 |
| 27 | Rz Love (D1) | USA | 52.00 | | +14.480 |
| 28 | Sky Thatcher (n/a) | USA | 57.00 | SB | +19.480 |