| 1 | Valentin Baum (B1) | GER | 42.66 | | - |
| 2 | Franziska Riedel (B2) | GER | 43.79 | | +1.130 |
| 3 | Tina Hemeltjen (A1) | GER | 43.86 | SB | +1.200 |
| 4 | Mia Meinig (B1) | GER | 44.77 | SB | +2.110 |
| 5 | Mara Fuhrmann (B2) | GER | 45.22 | SB | +2.560 |
| 6 | Johanna Trapp (B1) | GER | 45.69 | SB | +3.030 |
| 7 | Emma Schöler (C2) | GER | 45.71 | SB | +3.050 |
| 8 | Alicia Sommer (A2) | GER | 45.79 | SB | +3.130 |
| 9 | Johanna Richter (A1) | GER | 45.90 | SB | +3.240 |
| 10 | Sahra Michelle Henkel (B1) | GER | 46.45 | SB | +3.790 |
| 11 | Henriette Heß (C1) | GER | 46.81 | SB | +4.150 |
| 12 | Annalina Müller (B1) | GER | 47.04 | SB | +4.380 |
| 13 | Mika Louise Brandt (C2) | GER | 47.09 | | +4.430 |
| 14 | Alexa Dehs (C1) | GER | 47.21 | | +4.550 |
| 15 | Lena Mothes (C1) | GER | 47.62 | | +4.960 |
| 16 | Florentine Blümel (C2) | GER | 47.66 | | +5.000 |
| 17 | Lilli Malin Mittag (C1) | GER | 47.86 | | +5.200 |
| 18 | Josephine Helbig (C1) | GER | 48.38 | | +5.720 |
| 19 | Johanna Brückner (C1) | GER | 48.83 | | +6.170 |
| 20 | Claire Tuchscherer (C2) | GER | 48.88 | | +6.220 |
| 21 | Hannah Krompaß (C1) | GER | 49.17 | | +6.510 |
| 22 | Alina Kebsch (C2) | GER | 49.74 | | +7.080 |
| 23 | Livia Kosmala (D2) | GER | 49.94 | | +7.280 |
| 24 | July Seifert (C1) | GER | 50.21 | | +7.550 |
| 25 | Elisabeth Schweizer-Theodor (D2) | GER | 50.29 | | +7.630 |
| 26 | Linda Pauline Voland (D2) | GER | 50.78 | | +8.120 |
| 27 | Enola Seidl (C2) | GER | 51.17 | | +8.510 |
| 28 | Jasmin Schneider (D2) | GER | 52.95 | SB | +10.290 |
| 29 | Sara Andreas (D2) | GER | 54.21 | PB | +11.550 |
| 30 | Amelie Sophia Roßner (D2) | GER | 54.32 | | +11.660 |
| 31 | Ruby Winkler (D2) | GER | 58.45 | | +15.790 |
| 32 | Lee Xenia Langhof (D2) | GER | 1:00.81 | | +18.150 |
| DNS | Alexandra Lebsuch (C1) | GER | DNS | | - |
| DNS | Selina Schulze (D2) | GER | DNS | | - |