| 1 | Maaike Koomen (Neo) | NED | 42.84 | | - |
| 2 | Marieke Mulder (B2) | NED | 43.00 | | +0.160 |
| 3 | Manon Gremmen (Neo) | NED | 43.23 | PB | +0.390 |
| 4 | Nienke Kooistra (Senior) | NED | 43.41 | | +0.570 |
| 5 | Rigt Kalsbeek (Senior) | NED | 43.56 | PB | +0.720 |
| 6 | Esther Nauta (Senior) | NED | 43.58 | PB | +0.740 |
| 7 | Ellen van Vroonhoven (Senior) | NED | 43.63 | | +0.790 |
| 8 | Andrea Dalstra (A2) | NED | 43.66 | PB | +0.820 |
| 9 | Haike Verbree (Neo) | NED | 43.82 | | +0.980 |
| 10 | Iris Willemsen (Neo) | NED | 43.85 | SB | +1.010 |
| 11 | Corina van der Lei (Senior) | NED | 44.29 | | +1.450 |
| 12 | Eline Cox (A2) | NED | 44.51 | SB | +1.670 |
| 13 | Ellen Barendregt (Senior) | NED | 44.54 | PB | +1.700 |
| 14 | Mendy Schrijver (A1) | NED | 44.55 | | +1.710 |
| 15 | Erika Bremer (Neo) | NED | 44.59 | SB | +1.750 |
| 16 | Lotte Elfferich (A2) | NED | 44.86 | | +2.020 |
| 17 | Lotte Jongema (Neo) | NED | 45.09 | | +2.250 |
| 18 | Iris Smokers (Neo) | NED | 45.48 | | +2.640 |
| 19 | Arenda Steenhuis (Neo) | NED | 45.75 | | +2.910 |
| 20 | Jinke Miriam van Keulen (Neo) | NED | 45.83 | | +2.990 |
| 21 | Judith Krabbenborg (A2) | NED | 46.15 | | +3.310 |
| 22 | Jeske Hoogeboom (Neo) | NED | 46.82 | | +3.980 |
| 23 | Talita van Hartskamp (Neo) | NED | 46.84 | | +4.000 |
| 24 | Jip van Elst (A2) | NED | 47.41 | | +4.570 |
| 25 | Anke Weerts (Senior) | NED | 47.59 | | +4.750 |
| 26 | Anne Lambrechts (Neo) | NED | 47.89 | | +5.050 |
| 27 | Nicolle van Rijswijk (A2) | NED | 48.38 | | +5.540 |
| 28 | Adinda Mieras (Senior) | NED | 48.54 | | +5.700 |
| 29 | Martine Schouten Hoogendijk (Senior) | NED | 49.29 | SB | +6.450 |
| 30 | Heleen Schuch (A2) | NED | 49.77 | | +6.930 |
| 31 | Geke Kloosterziel (Neo) | NED | 49.93 | | +7.090 |
| 32 | Maud van Goor (Neo) | NED | 50.21 | | +7.370 |
| 33 | Fenna Visser (A2) | NED | 50.59 | SB | +7.750 |
| 34 | Nienke Schuur (A1) | NED | 51.78 | | +8.940 |
| 35 | Justine Eerden (Neo) | NED | 51.94 | | +9.100 |
| 36 | Jip Spel (Neo) | NED | 54.88 | | +12.040 |
| 37 | Liza van Kapel (Neo) | NED | 55.78 | | +12.940 |
| 38 | Maite Knaap (Neo) | NED | 57.21 | | +14.370 |
| DQ | Francine Kronenberg (Senior) | NED | DQ | | - |