| 1 | Sacha van der Weide (Neo) | NED | 39.94 | SB | - |
| 2 | Jillian Knook (A2) | NED | 40.04 | SB | +0.100 |
| 3 | Lotte Groenen (A2) | NED | 40.05 | SB | +0.110 |
| 4 | Meike Veen (A2) | NED | 40.48 | | +0.540 |
| 5 | Romée Ebbinge (Senior) | NED | 40.56 | SB | +0.620 |
| 6 | Sanne Westra (Neo) | NED | 40.61 | SB | +0.670 |
| 7 | Sterre van Schaik (A2) | NED | 40.88 | SB | +0.940 |
| 8 | Sofie Bouw (A2) | NED | 40.99 | SB | +1.050 |
| 9 | Isa Leroy (A1) | NED | 41.08 | SB | +1.140 |
| 10 | Rosalie van Vliet (A1) | NED | 41.36 | SB | +1.420 |
| 11 | Jet Fransen (A2) | NED | 41.42 | PB | +1.480 |
| 12 | Marika de Glee (B2) | NED | 41.46 | SB | +1.520 |
| 13 | Patricia Koot (A2) | NED | 41.58 | SB | +1.640 |
| 14 | Milou de Lange (A1) | NED | 41.62 | SB | +1.680 |
| 15 | Maaike Koelewijn (B2) | NED | 41.79 | SB | +1.850 |
| 16 | Sietske van der Meer (Neo) | NED | 41.86 | SB | +1.920 |
| 17 | Janna Bolsius (A2) | NED | 41.88 | | +1.940 |
| 18 | Sanne Oosterwijk (A2) | NED | 41.89 | SB | +1.950 |
| 19 | Vera van Ditshuizen (Neo) | NED | 41.96 | SB | +2.020 |
| 20 | Jildou Schaaf (Neo) | NED | 42.06 | SB | +2.120 |
| 21 | Elbrich Nicolay (A1) | NED | 42.17 | SB | +2.230 |
| 22 | Jasmijn Veenhuis (B2) | NED | 42.34 | SB | +2.400 |
| 23 | Elanne de Vries (A1) | NED | 42.43 | SB | +2.490 |
| 24 | Susanne Prins (A2) | NED | 42.51 | SB | +2.570 |
| 25 | Lianne van Gammeren (A1) | NED | 42.62 | SB | +2.680 |
| 26 | Jasmijn Soet (Neo) | NED | 42.80 | SB | +2.860 |
| 27 | Tosca Mulder (Neo) | NED | 42.89 | SB | +2.950 |
| 28 | Liotte Smits (Neo) | NED | 42.93 | SB | +2.990 |
| 29 | Emma Noz (A2) | NED | 42.97 | SB | +3.030 |
| 30 | Evi de Ruiter (B2) | NED | 43.01 | SB | +3.070 |
| 31 | Tijn Borst (A1) | NED | 43.05 | SB | +3.110 |
| 32 | Maud Biezen (B2) | NED | 43.08 | SB | +3.140 |
| 33 | Britt Breider (B2) | NED | 43.12 | SB | +3.180 |
| 34 | Fleur Smits (B2) | NED | 43.36 | | +3.420 |
| Sharissa Oussoren (B2) | NED | 43.36 | SB | +3.420 |
| 36 | Quinty Hersi (B1) | NED | 43.43 | SB | +3.490 |
| 37 | Sara ter Hart (B2) | NED | 43.69 | SB | +3.750 |
| 38 | Emma Kole (Neo) | NED | 43.79 | | +3.850 |
| 39 | Ilse Bierma (B2) | NED | 43.83 | SB | +3.890 |
| 40 | Ymke Wubben (B1) | NED | 44.29 | SB | +4.350 |
| 41 | Maud Kempenaar (B1) | NED | 45.49 | SB | +5.550 |
| 42 | Melle Boeijen (B2) | NED | 45.52 | SB | +5.580 |
| 43 | Amber Koetsier (B1) | NED | 45.68 | SB | +5.740 |
| 44 | Floortje Bregman (B2) | NED | 46.16 | SB | +6.220 |
| 45 | Maria Procópio Dias (C2) | POR | 46.40 | SB | +6.460 |
| 46 | Isa Oudenes (B1) | NED | 46.41 | SB | +6.470 |
| 47 | Leonor Almodôvar Ladeira (B1) | POR | 47.21 | SB | +7.270 |
| 48 | Clara Correia Cruz Pereira (B1) | POR | 48.62 | SB | +8.680 |
| 49 | Ana Rita Andrade de Freitas (B2) | POR | 51.92 | SB | +11.980 |
| 50 | Matilde Sofia Oliveira Ferreira (n/a) | POR | 52.34 | SB | +12.400 |
| DQ | Janine Garretsen (B2) | NED | DQ | | - |