| 1 | Cynthia Zetz (B2) | NED | 46.64 | SB | - |
| 2 | Marieke van Wijngaarden (Senior) | NED | 46.74 | | +0.100 |
| 3 | Natasja van Wijk (B1) | NED | 47.04 | | +0.400 |
| 4 | Christel van der Made (M30) | NED | 47.05 | | +0.410 |
| 5 | Petra de Boer-Grimbergen (M35) | NED | 47.34 | SB | +0.700 |
| 6 | Danielle Douma (B1) | NED | 47.65 | | +1.010 |
| 7 | Elaine Jansen (C2) | NED | 47.74 | | +1.100 |
| 8 | Aimee Stroink (M30) | NED | 47.79 | SB | +1.150 |
| 9 | Janet Dunning (M35) | NED | 48.15 | SB | +1.510 |
| 10 | Berdy Kars (Senior) | NED | 48.19 | | +1.550 |
| 11 | Tessa Keur (M35) | NED | 48.45 | | +1.810 |
| 12 | Heidi de Geus (M35) | NED | 48.82 | | +2.180 |
| 13 | Marjan Muis (M45) | NED | 49.62 | | +2.980 |
| 14 | Roos Lantink (Senior) | NED | 50.39 | | +3.750 |
| Josine de Winter (Senior) | NED | 50.39 | SB | +3.750 |
| 16 | Wendy de Graaf (M30) | NED | 50.69 | | +4.050 |
| 17 | Marjolein van Waes-van der Krogt (M30) | NED | 50.72 | SB | +4.080 |
| 18 | Aaltje Kreuning (M40) | NED | 51.00 | PB | +4.360 |
| Kaylee Snijders (B1) | NED | 51.00 | | +4.360 |
| 20 | Astrid Janssen (M45) | NED | 51.44 | | +4.800 |
| 21 | Emelie Sweerts (M40) | NED | 52.04 | | +5.400 |
| 22 | Maxine Gorter (C2) | NED | 52.64 | | +6.000 |
| 23 | Yvonne van der Voort (M30) | NED | 52.84 | | +6.200 |
| 24 | Miranda Hulst (M40) | NED | 53.15 | | +6.510 |
| 25 | Erica Della Guardia-de Jong (M35) | NED | 53.71 | | +7.070 |
| 26 | Ellen Kok (M40) | NED | 54.08 | | +7.440 |
| 27 | Mirjam Brinkman (Senior) | NED | 54.62 | PB | +7.980 |
| 28 | Inge de Nijs (M40) | NED | 55.68 | | +9.040 |
| 29 | Daphne van der Lans (Neo) | NED | 56.37 | | +9.730 |
| 30 | Sharon Douma (C1) | NED | 56.38 | | +9.740 |
| 31 | Monique Pastoor-Kreuni (M50) | NED | 56.48 | | +9.840 |
| 32 | Maaike Verbree (Senior) | NED | 57.34 | SB | +10.700 |
| 33 | Jacqueline Verbruggen (M40) | NED | 57.55 | SB | +10.910 |
| 34 | Carolien van Rees (M50) | NED | 58.75 | SB | +12.110 |
| 35 | Inge Muis (n/a) | NED | 1:00.75 | PB | +14.110 |
| 36 | Jill Soedarso (B1) | NED | 1:02.34 | | +15.700 |
| DNS | Chantal Brugmans (Neo) | NED | DNS | | - |
| DQ | Marike van der Schaaf (M40) | NED | DQ | | - |
| DNS | Anna Groenewold (Senior) | NED | DNS | | - |