| 1 | Jeike Juk (Neo) | NED | 49.15 | | - |
| 2 | Jenny van der Velde (Senior) | NED | 49.78 | | +0.630 |
| 3 | Judith Loopers (Senior) | NED | 52.79 | | +3.640 |
| 4 | Saskia van der Velde (Senior) | NED | 54.12 | SB | +4.970 |
| 5 | Nynke Ant Annema (Senior) | NED | 55.86 | PB | +6.710 |
| 6 | Marjet Janssen (Senior) | NED | 56.02 | | +6.870 |
| 7 | Fleur Mollema (Neo) | NED | 57.07 | SB | +7.920 |
| 8 | Isabel Stribos (Senior) | NED | 59.29 | | +10.140 |
| 9 | Fimke Anna Giliam (Neo) | NED | 59.57 | | +10.420 |
| 10 | Klaske Terpstra (Neo) | NED | 1:01.16 | | +12.010 |
| 11 | Hedwig Fossen (Neo) | NED | 1:01.18 | | +12.030 |
| 12 | Anne Blanken (Senior) | NED | 1:01.82 | SB | +12.670 |
| 13 | Lotte Tenkink (Neo) | NED | 1:02.23 | | +13.080 |
| 14 | Lisanne van der Werf (Neo) | NED | 1:02.99 | | +13.840 |
| 15 | Adrianne de Roo (Neo) | NED | 1:06.17 | | +17.020 |
| 16 | Merel Hage (Senior) | NED | 1:13.81 | PB | +24.660 |
| 17 | Iris van der Vegte (Senior) | NED | 1:14.56 | | +25.410 |
| 18 | Femke Salverda (Senior) | NED | 1:17.42 | PB | +28.270 |
| DQ | Amarins Jager (Senior) | NED | DQ | | - |