| 1 | Emma Noz (B2) | NED | 44.90 | | - |
| 2 | Liesbeth Veen-Milatz (Senior) | NED | 45.76 | SB | +0.860 |
| 3 | Patou de Haas (A2) | NED | 46.59 | SB | +1.690 |
| 4 | Bodine Vollering (A1) | NED | 47.79 | SB | +2.890 |
| 5 | Regina Bruins (Senior) | NED | 48.44 | PB | +3.540 |
| 6 | Roos-Marie van Gerven (M45) | NED | 48.68 | SB | +3.780 |
| 7 | Pauline Tas (D2) | NED | 49.03 | SB | +4.130 |
| 8 | Marianne de Neeling (M60) | NED | 49.44 | | +4.540 |
| 9 | Faye Duijvendijk (C1) | NED | 49.88 | | +4.980 |
| 10 | Cheyenne van der Breggen (Neo) | NED | 50.22 | | +5.320 |
| 11 | Fiore Koutstaal (D1) | NED | 50.54 | | +5.640 |
| 12 | Dianne Vos (A2) | NED | 50.59 | SB | +5.690 |
| 13 | Lisanne Vreugdenhil (D2) | NED | 50.79 | SB | +5.890 |
| 14 | Julia van den Berg (Neo) | NED | 50.96 | SB | +6.060 |
| 15 | Cato Slegtenhorst (D1) | NED | 52.27 | SB | +7.370 |
| 16 | Iris van den Helder (C2) | NED | 52.60 | PB | +7.700 |
| 17 | Krista Geluk (A2) | NED | 54.33 | SB | +9.430 |
| 18 | Sanne Plag (D1) | NED | 1:04.51 | | +19.610 |
| 19 | Hester Peters (n/a) | NED | 1:17.65 | PB | +32.750 |
| 20 | Merel Bergenhenegouwen (n/a) | NED | 1:24.68 | SB | +39.780 |
| 21 | Anushree Kandge (n/a) | NED | 1:54.09 | SB | +1.09.190 |