| 1 | Felicia Louhenapessy (Neo) | NED | 46.10 | SB | - |
| 2 | Marlijn van Aanhold (A2) | NED | 46.68 | PB | +0.580 |
| 3 | Dorian Schoonderwoerd (M45) | NED | 47.37 | SB | +1.270 |
| 4 | Isa Meerveld (B2) | NED | 48.05 | | +1.950 |
| 5 | Veerle van der Does (B1) | NED | 48.56 | | +2.460 |
| 6 | Senna Hoornstra (B2) | NED | 50.17 | PB | +4.070 |
| 7 | Merlinde de Bie (A1) | NED | 51.22 | | +5.120 |
| 8 | Martine van der Herberg (Senior) | NED | 51.55 | SB | +5.450 |
| 9 | Eveline Zijderveld (Neo) | NED | 52.56 | SB | +6.460 |
| 10 | Medea Schalkwijk (B1) | NED | 53.99 | SB | +7.890 |
| 11 | Nouk van Doornik (A2) | NED | 54.00 | SB | +7.900 |
| 12 | Mirte Verweij (D2) | NED | 54.37 | | +8.270 |
| 13 | Tirsa Hagoort (C1) | NED | 55.96 | | +9.860 |
| 14 | Emma Visser (C2) | NED | 1:02.38 | | +16.280 |
| 15 | Anne Maaike van Selm (B1) | NED | 1:03.03 | | +16.930 |
| 16 | Lotte Smits (C2) | NED | 1:05.14 | | +19.040 |
| 17 | Esmée Blaauw (D1) | NED | 1:08.01 | | +21.910 |
| 18 | Hannah Jalvingh (D1) | NED | 1:12.23 | | +26.130 |
| 19 | Josje van der Neut (E1) | NED | 1:12.26 | | +26.160 |
| 20 | Renee Landgraf (B1) | NED | 1:13.56 | SB | +27.460 |
| 21 | Stella Smit (D1) | NED | 1:24.52 | | +38.420 |
| DNS | Femke Jager (Senior) | NED | DNS | | - |