| 1 | Chrysta Rands (Senior) | USA | 39.96 | | - |
| 2 | McKenzie Browne (Senior) | USA | 40.02 | | +0.060 |
| 3 | Vera Güntert (Senior) | SUI | 40.31 | | +0.350 |
| 4 | Anna Quinn (Neo) | USA | 41.90 | | +1.940 |
| 5 | Lindsey Woodbury (A2) | USA | 42.32 | | +2.360 |
| 6 | Laura Michelle Rodriguez (B1) | COL | 42.38 | | +2.420 |
| 7 | Gemma Cooper (Senior) | GBR | 42.44 | PB | +2.480 |
| 8 | Thalia Staehle (A1) | USA | 42.63 | | +2.670 |
| 9 | Abigail Hailey Sorenson (A2) | USA | 42.99 | | +3.030 |
| 10 | Shruti Kotwal (M30) | IND | 43.04 | | +3.080 |
| 11 | Larissa Paes (Senior) | BRA | 43.38 | | +3.420 |
| 12 | Marley Soldan (C2) | USA | 44.01 | SB | +4.050 |
| 13 | Ann-Marie Lindqvist (Senior) | SWE | 44.28 | | +4.320 |
| 14 | Ella Teeples (D2) | USA | 47.00 | | +7.040 |
| 15 | Mariana Franco (B2) | COL | 47.34 | | +7.380 |
| 16 | Avery Hayes (D2) | USA | 51.36 | | +11.400 |
| 17 | Scarelette Gramlich Thatcher (E2) | USA | 52.58 | | +12.620 |
| DQ | Isabela Vargas Rodriguez (C2) | COL | DQ | | - |