| 1 | Kimi Goetz (Senior) | USA | 38.33 | | - |
| 2 | McKenzie Browne (Senior) | USA | 38.64 | PB | +0.310 |
| 3 | Erin Jackson (Senior) | USA | 38.75 | | +0.420 |
| 4 | Chrysta Rands-Evans (Senior) | USA | 39.19 | SB | +0.860 |
| 5 | Greta Myers (A2) | USA | 39.99 | | +1.660 |
| 6 | Mia Kilburg-Manganello (Senior) | USA | 40.86 | | +2.530 |
| 7 | Giorgia Birkeland (Neo) | USA | 41.81 | | +3.480 |
| 8 | Laura Michelle Rodriguez (B2) | COL | 41.93 | | +3.600 |
| 9 | Brittany Bowe (Senior) | USA | 42.14 | | +3.810 |
| 10 | Natalia Valencia (Senior) | COL | 42.49 | | +4.160 |
| 11 | Lindsey Woodbury (Neo) | USA | 42.59 | | +4.260 |
| 12 | Shruti Kotwal (M30) | IND | 42.78 | NRM | +4.450 |
| 13 | Isabela Vargas Rodriguez (B1) | COL | 43.80 | | +5.470 |
| 14 | Karen Siatama (B2) | COL | 45.16 | | +6.830 |
| 15 | Scarelette Gramlich Thatcher (D1) | USA | 51.14 | SB | +12.810 |
| DNS | Sofia Prosvirnova (Senior) | RUS | DNS | | - |
| DNS | Ilsa Melin Sandvig Shobe (Senior) | USA | DNS | | - |
| DNS | Rz Love (C1) | USA | DNS | | - |