| 1 | Natasa Gakovic (Neo) | USA | 43.12 | SB | - |
| 2 | Evie Forrer (B2) | USA | 43.72 | | +0.600 |
| 3 | Nastasja Radulovic (Neo) | USA | 44.93 | | +1.810 |
| 4 | Valery Molinares (C2) | USA | 46.36 | PB | +3.240 |
| 5 | Kassidy González Mcglauchlen (B1) | USA | 49.23 | SB | +6.110 |
| 6 | Libby Anderson (C2) | USA | 49.40 | SB | +6.280 |
| 7 | Tillie Quant (D1) | USA | 50.05 | PB | +6.930 |
| 8 | Hazel Joyer (C1) | USA | 51.07 | | +7.950 |
| 9 | Mary Murphy (M35) | USA | 51.66 | SB | +8.540 |
| 10 | Isabel Tschida (C1) | USA | 52.66 | PB | +9.540 |
| Luciana Molinares Rodriguez (E2) | USA | 52.66 | PB | +9.540 |
| 12 | Alice Chamberlain (D1) | USA | 54.18 | SB | +11.060 |
| 13 | Kelly Bennett-Komornicka (M45) | USA | 55.39 | | +12.270 |
| 14 | Madeleine Schlichting (C1) | CAN | 1:01.33 | | +18.210 |
| 15 | Katrina Bjergaard (D2) | USA | 1:03.62 | | +20.500 |
| 16 | Julia Dallmeyer (n/a) | USA | 1:04.03 | PB | +20.910 |
| 17 | Saehee Lippert (n/a) | USA | 1:04.13 | PB | +21.010 |
| 18 | Isabella Ramos Young (E1) | USA | 1:09.60 | | +26.480 |
| 19 | Zoe Barron-Eshleman (n/a) | USA | 1:10.90 | PB | +27.780 |
| 20 | Savannah Bjergaard (n/a) | USA | 1:18.26 | PB | +35.140 |
| 21 | Evangeline Milles (F2) | USA | 1:19.91 | | +36.790 |