| 1 | Maria Victoria Rodriguez (Senior) | ARG | 39.55 | | - |
| 2 | Anna Quinn (Senior) | USA | 41.03 | | +1.480 |
| 3 | Abigél Mercs (Neo) | HUN | 41.34 | PB | +1.790 |
| 4 | Ilsa Melin Sandvig Shobe (Senior) | USA | 42.07 | | +2.520 |
| 5 | Amy Acosta (Senior) | ECU | 42.16 | SB | +2.610 |
| 6 | Libby Williams (Neo) | USA | 42.31 | | +2.760 |
| 7 | Karen Lizeth Siatama Valencia (Neo) | COL | 42.38 | SB | +2.830 |
| 8 | Natalia Valencia (Senior) | COL | 42.40 | | +2.850 |
| 9 | Natascha Lindenskov (Neo) | DEN | 42.81 | PB | +3.260 |
| 10 | Larissa Paes (M30) | BRA | 42.96 | | +3.410 |
| 11 | Shruti Kotwal (M30) | IND | 43.20 | | +3.650 |
| 12 | Cynthia Jennings (Senior) | USA | 44.04 | | +4.490 |
| 13 | Rebecca Simmons (M35) | USA | 44.45 | SB | +4.900 |
| DNS | Erica Orndorff (n/a) | USA | DNS | | - |