| 1 | Erin Jackson (Senior) | USA | 37.88 | SB | - |
| 2 | Brittany Bowe (Senior) | USA | 38.20 | SB | +0.320 |
| 3 | Sarah Warren (Senior) | USA | 38.44 | SB | +0.560 |
| 4 | McKenzie Browne (Senior) | USA | 38.78 | SB | +0.900 |
| 5 | Greta Myers (Neo) | USA | 38.82 | SB | +0.940 |
| 6 | Sofia Prosvirnova (Senior) | DEN | 39.44 | NR | +1.560 |
| 7 | Chrysta Rands-Evans (Senior) | USA | 39.86 | SB | +1.980 |
| 8 | Mia Manganello (Senior) | USA | 40.20 | SB | +2.320 |
| 9 | Anna Quinn (Senior) | USA | 40.82 | | +2.940 |
| 10 | Laura Michelle Rodriguez (A1) | COL | 41.29 | SB | +3.410 |
| 11 | Libby Williams (A2) | USA | 41.69 | SB | +3.810 |
| 12 | Giorgia Birkeland (Neo) | USA | 42.10 | | +4.220 |
| 13 | Isabela Vargas Rodriguez (B2) | COL | 42.39 | PB | +4.510 |
| 14 | Abigél Mercs (Neo) | HUN | 42.92 | SB | +5.040 |
| Shruti Kotwal (M30) | IND | 42.92 | SB | +5.040 |
| 16 | Ilsa Melin Sandvig Shobe (Senior) | USA | 42.94 | SB | +5.060 |
| 17 | Ella Teeples (C2) | USA | 43.19 | | +5.310 |
| 18 | Karen Siatama (A1) | COL | 44.37 | | +6.490 |
| 19 | Selena Su (B1) | USA | 45.39 | SB | +7.510 |
| 20 | Scarelette Gramlich Thatcher (D2) | USA | 48.72 | PB | +10.840 |
| 21 | Kathy Feinberg (M60) | USA | 57.78 | SB | +19.900 |