| 1 | Kimi Goetz (Senior) | USA | 38.30 | | - |
| 2 | McKenzie Browne (Senior) | USA | 39.20 | | +0.900 |
| 3 | Chrysta Rands-Evans (Senior) | USA | 39.69 | | +1.390 |
| 4 | Anna Quinn (Neo) | USA | 40.88 | | +2.580 |
| 5 | Laura Michelle Rodriguez (B2) | COL | 42.20 | | +3.900 |
| 6 | Shruti Kotwal (M30) | IND | 42.21 | NR | +3.910 |
| 7 | Lindsey Woodbury (Neo) | USA | 42.43 | | +4.130 |
| 8 | Thalia Staehle (A2) | USA | 42.80 | | +4.500 |
| 9 | Larissa Paes (Senior) | BRA | 42.81 | | +4.510 |
| 10 | Ilsa Melin Sandvig Shobe (Senior) | USA | 42.98 | | +4.680 |
| 11 | Rebecca Simmons (M30) | USA | 44.23 | | +5.930 |
| 12 | Ella Teeples (C1) | USA | 44.68 | | +6.380 |
| 13 | Karen Siatama (B2) | COL | 44.71 | SB | +6.410 |
| 14 | Rz Love (C1) | USA | 46.11 | | +7.810 |
| 15 | Avery Hayes (C1) | USA | 47.50 | | +9.200 |
| 16 | Scarelette Gramlich Thatcher (D1) | USA | 52.35 | | +14.050 |
| DQ | Isabela Vargas Rodriguez (B1) | COL | DQ | | - |