| 1 | Kimi Goetz (Senior) | USA | 38.30 | | - |
| 2 | Erin Jackson (Senior) | USA | 38.51 | | +0.210 |
| 3 | Brianna Bocox (Senior) | USA | 39.18 | | +0.880 |
| 4 | Paige Schwartzburg (Senior) | USA | 39.76 | | +1.460 |
| 5 | Mia Kilburg-Manganello (Senior) | USA | 40.19 | | +1.890 |
| 6 | Sarah Warren (Senior) | USA | 40.35 | | +2.050 |
| 7 | McKenzie Browne (Senior) | USA | 40.41 | | +2.110 |
| 8 | Jamie Jurak (Senior) | USA | 41.83 | | +3.530 |
| 9 | Giorgia Birkeland (A2) | USA | 42.13 | SB | +3.830 |
| 10 | Piper Yde (B1) | USA | 42.15 | | +3.850 |
| 11 | Jamie Lindquist-Nielson (Neo) | USA | 42.80 | | +4.500 |
| 12 | Lindsey Woodbury (A1) | USA | 43.18 | | +4.880 |
| 13 | Natalia Valencia (Neo) | COL | 43.59 | | +5.290 |
| 14 | Valentina Espinosa (Neo) | ARG | 43.64 | | +5.340 |
| 15 | Laura Michelle Rodriguez (C2) | COL | 44.72 | | +6.420 |
| 16 | Ema Hinckley (Neo) | POR | 44.78 | | +6.480 |
| 17 | Ilsa Melin Sandvig Shobe (Neo) | USA | 44.89 | | +6.590 |