| 1 | Erin Jackson (Senior) | USA | 38.40 | | - |
| 2 | Brianna Bocox (Neo) | USA | 39.12 | | +0.720 |
| 3 | Keira Poelzer (Senior) | CAN | 40.47 | | +2.070 |
| 4 | Esther Munoz (Neo) | USA | 41.47 | | +3.070 |
| 5 | Dessie Weigel (Senior) | USA | 42.12 | | +3.720 |
| 6 | Jenni Kukkonen (Senior) | FIN | 42.44 | | +4.040 |
| 7 | Dalia Soberanis (Neo) | GUA | 43.28 | NR | +4.880 |
| 8 | Jamie Lindquist-Nielson (Neo) | USA | 43.66 | SB | +5.260 |
| 9 | Rebecca Simmons (M30) | USA | 43.70 | | +5.300 |
| 10 | Shruti Kotwal (Senior) | IND | 44.81 | SB | +6.410 |
| 11 | Ema Hinckley (A2) | POR | 45.49 | NRJ, NR | +7.090 |
| 12 | Laura Michelle Rodriguez (C1) | COL | 47.74 | | +9.340 |
| 13 | Manuela Escobar Ballen (D1) | COL | 52.26 | | +13.860 |
| 14 | Ana Sofia Santos (D2) | COL | 55.62 | | +17.220 |
| 15 | Isabela Higuita (C1) | COL | 1:00.03 | | +21.630 |
| 16 | Vanessa Daza (Neo) | COL | 1:01.13 | | +22.730 |
| 17 | Isabela Vargas Rodriguez (D2) | COL | 1:09.58 | | +31.180 |
| 18 | Margarita Torres (F2) | COL | 1:29.26 | | +50.860 |