| 1 | Ireen Wüst (Senior) | NED | 38.55 | | - |
| 2 | Ivanie Blondin (Senior) | CAN | 38.83 | SB | +0.280 |
| 3 | Chong Pei (Neo) | CHN | 38.86 | | +0.310 |
| 4 | Antoinette de Jong (Senior) | NED | 38.93 | | +0.380 |
| 5 | Lotte van Beek (Senior) | NED | 39.15 | | +0.600 |
| 6 | Francesca Lollobrigida (Senior) | ITA | 39.25 | | +0.700 |
| 7 | Dan Li (Senior) | CHN | 39.26 | | +0.710 |
| 8 | Ida Njåtun (Senior) | NOR | 39.29 | | +0.740 |
| 9 | Carlijn Achtereekte (Senior) | NED | 39.32 | | +0.770 |
| 10 | Kali Christ (Senior) | CAN | 39.34 | | +0.790 |
| 11 | Abigail Mccluskey (Neo) | CAN | 39.52 | SB | +0.970 |
| 12 | Maddison Pearman (Neo) | CAN | 39.71 | | +1.160 |
| 13 | Elena Sokhryakova (Senior) | RUS | 39.78 | | +1.230 |
| 14 | Joy Beune (Neo) | NED | 39.80 | | +1.250 |
| 15 | Carolina Hiller (Neo) | CAN | 39.88 | | +1.330 |
| 16 | Courtney Shmyr (Senior) | CAN | 40.17 | | +1.620 |
| 17 | Marina Zueva (Senior) | BLR | 40.27 | | +1.720 |
| 18 | Isabelle Weidemann (Neo) | CAN | 40.45 | | +1.900 |
| 19 | Valerie Maltais (Senior) | CAN | 40.63 | | +2.080 |
| 20 | Jiaying Tao (Senior) | CHN | 40.67 | | +2.120 |
| 21 | Qi Yin (Senior) | CHN | 40.75 | | +2.200 |
| 22 | Saskia Alusalu (Senior) | EST | 40.85 | NR | +2.300 |
| 23 | Lindsey Kent (Neo) | CAN | 40.93 | | +2.380 |
| 24 | Esmee Visser (Neo) | NED | 41.59 | SB | +3.040 |
| 25 | Hayleigh Bell (Neo) | CAN | 42.44 | | +3.890 |
| 26 | Danielle Wallish (Senior) | CAN | 42.53 | PB | +3.980 |
| 27 | Yasmine Uden (A2) | CAN | 44.28 | PB | +5.730 |