| 1 | Rebekah Bradford-Plath (M35) | USA | 39.90 | | - |
| 2 | Yu-Ting Huang (Senior) | TPE | 39.93 | NR | +0.030 |
| 3 | Maria Victoria Rodriguez (Senior) | ARG | 40.50 | NR | +0.600 |
| 4 | Franchesca Pasquarella (Neo) | USA | 40.67 | | +0.770 |
| 5 | Erin Bartlett (Senior) | USA | 40.78 | | +0.880 |
| 6 | Brianna Bocox (Neo) | USA | 40.79 | | +0.890 |
| 7 | Chrysta Rands (Neo) | USA | 41.82 | | +1.920 |
| 8 | Esther Munoz (Neo) | USA | 42.05 | | +2.150 |
| 9 | Briana Kramer (Senior) | USA | 42.12 | SB | +2.220 |
| 10 | Mariah Richardson (Senior) | USA | 42.17 | | +2.270 |
| 11 | Jamie Lindquist-Nielson (A2) | USA | 43.05 | | +3.150 |
| 12 | Valentina Espinosa (A2) | COL | 45.08 | | +5.180 |
| 13 | Laura Gomez Quintero (Senior) | COL | 45.52 | | +5.620 |
| 14 | Courtney Anderson (A2) | USA | 48.01 | | +8.110 |
| 15 | Tessa Lessner (C1) | USA | 49.34 | | +9.440 |
| DNS | Jenni Kukkonen (Senior) | FIN | DNS | | - |