| 1 | Ying-Chu Chen (Senior) | TPE | 38.51 | SB | - |
| 2 | Jenna Larter (Senior) | CAN | 38.74 | SB | +0.230 |
| 3 | Carolina Hiller-Donnelly (Senior) | CAN | 39.01 | SB | +0.500 |
| 4 | Brooklyn McDougall (Senior) | CAN | 39.12 | SB | +0.610 |
| 5 | Maddison Pearman (Senior) | CAN | 39.65 | SB | +1.140 |
| 6 | Alexa Scott (Senior) | CAN | 39.84 | SB | +1.330 |
| 7 | Katja Franzen (M35) | GER | 40.27 | SB | +1.760 |
| 8 | Abigail Mccluskey (Senior) | CAN | 40.31 | SB | +1.800 |
| 9 | Zhiyuan Wan (B1) | CHN | 40.32 | SB | +1.810 |
| 10 | Isabelle Champagne (A1) | CAN | 40.70 | SB | +2.190 |
| 11 | Victoria Goplen (Senior) | CAN | 40.78 | SB | +2.270 |
| 12 | Tatiana de Vlieg (A2) | CAN | 40.84 | SB | +2.330 |
| 13 | Darby Beeson (Neo) | CAN | 40.86 | PB | +2.350 |
| 14 | Sijia.05 Chen (Neo) | CHN | 41.16 | SB | +2.650 |
| 15 | Emma Langstrom (Senior) | CAN | 41.39 | SB | +2.880 |
| 16 | Veronika Antosova (Senior) | CZE | 41.50 | SB | +2.990 |
| 17 | Yimin Wang (A2) | CHN | 41.57 | SB | +3.060 |
| 18 | Zhixian.09 Wan (B1) | CHN | 41.60 | PB | +3.090 |
| 19 | Maya Lueders (Neo) | CAN | 41.63 | SB | +3.120 |
| 20 | Robyn Salie (Neo) | CAN | 41.64 | SB | +3.130 |
| 21 | Aoife Marshall (Neo) | CAN | 41.73 | SB | +3.220 |
| 22 | Laura Hall (Neo) | CAN | 41.82 | SB | +3.310 |
| 23 | Yuanyuan Zhang (Neo) | CHN | 42.03 | SB | +3.520 |
| 24 | Willa Dowse (B1) | CAN | 42.33 | PB | +3.820 |
| 25 | Rachel Mallard (Neo) | CAN | 42.47 | SB | +3.960 |
| 26 | Rui Zhang (A1) | CHN | 42.59 | PB | +4.080 |
| 27 | Serena Dallaire (Neo) | CAN | 42.90 | SB | +4.390 |
| 28 | Mia Tetrault (C2) | CAN | 43.69 | SB | +5.180 |
| 29 | Ranja Ezzi (M40) | SWE | 43.88 | SB | +5.370 |
| 30 | Daria Vogt (Neo) | CAN | 44.46 | SB | +5.950 |
| 31 | Gemma Cooper (Senior) | GBR | 44.52 | SB | +6.010 |
| 32 | Evyn Carr (B1) | CAN | 44.93 | SB | +6.420 |
| 33 | Rebecca Currie (B1) | CAN | 44.99 | SB | +6.480 |
| 34 | Cassandra Currie (A2) | CAN | 45.19 | SB | +6.680 |
| 35 | Julianne Lazzari (A1) | CAN | 45.30 | PB | +6.790 |
| 36 | Sophia Cairo Martinez (B2) | CAN | 45.49 | SB | +6.980 |
| 37 | Tess Overvelde (Neo) | CAN | 46.23 | SB | +7.720 |
| 38 | Isla Mason (B1) | CAN | 46.74 | SB | +8.230 |
| 39 | Isabel Erasmus (Neo) | CAN | 46.82 | SB | +8.310 |
| 40 | Alina Frischknecht (Senior) | SUI | 47.07 | PB | +8.560 |
| 41 | Leah Crisp (A2) | AUS | 47.12 | SB | +8.610 |
| 42 | Jillian Reeves (Neo) | CAN | 47.15 | SB | +8.640 |
| 43 | Megan Vansickle (B2) | CAN | 47.59 | SB | +9.080 |
| 44 | Kayleigh Roberts (C2) | CAN | 48.08 | | +9.570 |
| 45 | Charlotte Perkins (B1) | CAN | 48.11 | SB | +9.600 |
| 46 | Elizabeth Rea (B2) | CAN | 48.18 | SB | +9.670 |
| 47 | Poppy Wheelhouse (C2) | CAN | 49.85 | SB | +11.340 |
| 48 | Norah Gillings (B1) | CAN | 50.29 | SB | +11.780 |
| 49 | Nora Nelly Solazzo (Neo) | SUI | 51.30 | SB | +12.790 |
| 50 | Diane Silzer (M65) | CAN | 51.85 | SB | +13.340 |
| 51 | Jan Zurcher (M70) | USA | 1:00.76 | SB | +22.250 |