| 1 | Cindy Vergeer (Neo) | NED | 41.73 | | - |
| 2 | Eline Bennis (B2) | NED | 42.83 | | +1.100 |
| 3 | Nadine Bruineberg (Senior) | NED | 43.23 | SB | +1.500 |
| 4 | Annabel Regtvoort (A1) | NED | 43.62 | SB | +1.890 |
| 5 | Juliette Salomons (B2) | NED | 43.90 | PB | +2.170 |
| 6 | Natasja Roest (C2) | NED | 43.91 | | +2.180 |
| 7 | Simone van der Star (B1) | NED | 43.93 | | +2.200 |
| 8 | Linda de Groot (C2) | NED | 44.15 | SB | +2.420 |
| 9 | Nynke de Jong (A1) | NED | 44.28 | | +2.550 |
| 10 | Marléne Vermeij (B1) | NED | 44.34 | PB | +2.610 |
| 11 | Renee Lauppe (C2) | NED | 44.51 | PB | +2.780 |
| 12 | Esther van Wijngaarden (B1) | NED | 44.65 | SB | +2.920 |
| 13 | Marloes Heus (Neo) | NED | 44.74 | | +3.010 |
| 14 | Marieke Mast (B2) | NED | 44.79 | SB | +3.060 |
| 15 | Katinka van Willenswaard (Neo) | NED | 45.37 | | +3.640 |
| 16 | Lotte van Eeken (Neo) | NED | 45.39 | | +3.660 |
| 17 | Marieke van Ede (B2) | NED | 45.51 | | +3.780 |
| 18 | Akke Pronk (B2) | NED | 45.57 | | +3.840 |
| 19 | Saskia Tersteeg (C1) | NED | 46.25 | SB | +4.520 |
| 20 | Marlous Prinzen (Senior) | NED | 46.55 | PB | +4.820 |
| 21 | Natalie Timmer (A2) | NED | 46.67 | SB | +4.940 |
| 22 | Sanne Rooimans (B1) | NED | 46.82 | SB | +5.090 |
| 23 | Yvonne van der Meent (A2) | NED | 46.86 | | +5.130 |
| 24 | Danique Vergeer (D2) | NED | 46.99 | SB | +5.260 |
| 25 | Tessa van der Neut (C1) | NED | 47.01 | | +5.280 |
| 26 | Roselieke Grendel (A1) | NED | 47.32 | | +5.590 |
| 27 | Floortje Mackay (D2) | NED | 47.33 | SB | +5.600 |
| 28 | Inge Mostert (D2) | NED | 47.74 | SB | +6.010 |
| 29 | Esther van Iren (B1) | NED | 47.79 | SB | +6.060 |
| Lotte.92 de Jong (B2) | NED | 47.79 | SB | +6.060 |
| 31 | Willemijn Cnossen (D2) | NED | 48.05 | | +6.320 |
| 32 | Belle de Gast (A1) | NED | 48.19 | | +6.460 |
| 33 | Maartje Olsthoorn (B1) | NED | 48.81 | | +7.080 |
| 34 | Lara Okkerman (B1) | NED | 48.88 | PB | +7.150 |
| 35 | Kim van der Veer (B2) | NED | 49.08 | SB | +7.350 |
| 36 | Inez van der Steen (C1) | NED | 49.12 | | +7.390 |
| 37 | Lisanne de Goeij (B2) | NED | 50.11 | PB | +8.380 |
| Fleur Olsthoorn (B1) | NED | 50.11 | PB | +8.380 |
| 39 | Kim Leliveld (B1) | NED | 50.30 | SB | +8.570 |
| 40 | Evelien Oosterom (B2) | NED | 50.34 | SB | +8.610 |
| 41 | Daphne Velzeboer (D2) | NED | 50.37 | | +8.640 |
| 42 | Joyce van Vliet (C2) | NED | 50.57 | SB | +8.840 |
| 43 | Margriet Zijderlaan (Senior) | NED | 51.44 | | +9.710 |
| 44 | Sabine Jobsen (B1) | NED | 53.08 | | +11.350 |
| 45 | Pernilla Scheelings (C1) | NED | 53.83 | PB | +12.100 |
| 46 | Iris Beijeman (D2) | NED | 53.91 | SB | +12.180 |
| 47 | Charlotte van Klooster (B1) | NED | 54.01 | | +12.280 |
| 48 | Daniëlle van Dam (C2) | NED | 55.34 | | +13.610 |
| 49 | Milou Nieuwenhuizen (D2) | NED | 55.50 | SB | +13.770 |
| 50 | Emma Vork (D2) | NED | 56.19 | SB | +14.460 |
| 51 | Emma Toor (E2) | NED | 57.16 | SB | +15.430 |
| 52 | Jolanda Meier (D2) | NED | 1:05.04 | SB | +23.310 |
| DNS | Benita de Man (B1) | NED | DNS | | - |