| 1 | Ainoa Carreno (A1) | ESP | 41.32 | NRJ, NR | - |
| 2 | Luisa Gonzalez Salazar (B1) | ESP | 41.39 | NRJ | +0.070 |
| 3 | Britt de Boer (Neo) | NED | 41.43 | PB | +0.109 |
| 4 | Jade Groenewoud (B1) | NED | 41.84 | SB | +0.527 |
| 5 | Famke Minnee (A1) | NED | 42.02 | | +0.705 |
| 6 | Jitte Gjaltema (Neo) | NED | 42.09 | SB | +0.777 |
| 7 | Amber Bartelds (Neo) | NED | 42.25 | | +0.938 |
| 8 | Dieke Hendriks (Neo) | NED | 42.49 | | +1.177 |
| 9 | Eva Schipper (A1) | NED | 42.70 | | +1.380 |
| 10 | Yasmine Bouaziz (B1) | NED | 43.01 | | +1.689 |
| 11 | Semmie Bakker (B1) | NED | 43.09 | | +1.778 |
| 12 | Renata Velthuijsen (Senior) | NED | 43.19 | | +1.869 |
| 13 | Sofie Bouw (C2) | NED | 43.24 | | +1.922 |
| 14 | Kayleigh ter Laak (A1) | NED | 43.25 | | +1.934 |
| 15 | Tess Postma (C2) | NED | 43.28 | | +1.961 |
| 16 | Evelieke Kool (A2) | NED | 43.29 | | +1.974 |
| 17 | Lara van Son (B1) | NED | 43.32 | | +2.002 |
| 18 | Judith Krabbenborg (Neo) | NED | 43.45 | | +2.137 |
| 19 | Saffron Nijenhuis (C2) | NED | 44.09 | | +2.775 |
| 20 | Tosca Mulder (B1) | NED | 44.17 | | +2.849 |
| 21 | Thirza Koers (B2) | NED | 44.25 | | +2.937 |
| 22 | Lotte Elfferich (Neo) | NED | 44.27 | | +2.953 |
| 23 | Iris Tiben (B2) | NED | 44.39 | | +3.073 |
| 24 | Isa Leroy (C1) | NED | 44.78 | SB | +3.468 |
| 25 | Jamie Palilaja (Neo) | IDN | 44.87 | | +3.557 |
| 26 | Juliana Ebbens (B2) | NED | 45.19 | | +3.875 |
| 27 | Emmy Pordon (Neo) | NED | 45.41 | SB | +4.098 |
| 28 | Senna Lamme (D2) | NED | 45.85 | | +4.530 |
| 29 | Fleur Brink (B1) | NED | 45.94 | | +4.625 |
| 30 | Zefina de Bruin (A1) | NED | 46.59 | | +5.269 |
| 31 | Luna Nieuwland (D2) | NED | 46.66 | SB | +5.346 |
| 32 | Roos Schieman (C2) | NED | 46.80 | | +5.485 |
| 33 | Britt Breider (D2) | NED | 46.91 | | +5.591 |
| 34 | Maud Salomons (C1) | NED | 47.14 | | +5.827 |
| 35 | Fleur Smits (D2) | NED | 47.25 | | +5.931 |
| 36 | Dana Smits (C2) | NED | 47.33 | | +6.018 |
| 37 | Esther van Leeuwen (Senior) | NED | 47.44 | SB | +6.125 |
| 38 | Kate Lynn Schouten (D2) | NED | 47.51 | | +6.197 |
| 39 | Lisa Hollander (C2) | NED | 47.52 | | +6.207 |
| 40 | Mimi van Hall (D2) | NED | 47.94 | | +6.619 |
| 41 | Isabella Talen (D1) | NED | 47.96 | | +6.644 |
| 42 | Anne-Fleur van der Lied (D2) | NED | 48.23 | | +6.912 |
| 43 | Luna van Polen (C1) | NED | 48.37 | | +7.053 |
| 44 | Rianne Tiben (C2) | NED | 48.45 | | +7.134 |
| 45 | Bo Meijer (B1) | NED | 48.76 | | +7.445 |
| 46 | Demi Bartelds (A1) | NED | 48.98 | | +7.664 |