| 1 | Anne Plat (Senior) | NED | 40.52 | | - |
| 2 | Elanne de Vries (A1) | NED | 41.14 | SB | +0.620 |
| 3 | Marije van der Spek (Neo) | NED | 41.20 | SB | +0.680 |
| 4 | Tessa Oudman (Senior) | NED | 41.49 | | +0.970 |
| 5 | Thirza Koers (Neo) | NED | 42.49 | SB | +1.970 |
| 6 | Charley Boekema (D1) | NED | 42.63 | SB | +2.110 |
| 7 | Fleur Hartveld (D2) | NED | 42.65 | SB | +2.130 |
| 8 | Esther Withagen (A2) | NED | 42.69 | | +2.170 |
| 9 | Elise Favre (B1) | NED | 42.97 | SB | +2.450 |
| 10 | Jasmin van der Terp (A1) | NED | 43.06 | SB | +2.540 |
| 11 | Martha Trude Brehmer (C1) | SWE | 43.73 | PB | +3.210 |
| 12 | Mayke Vriesinga (A1) | NED | 43.88 | | +3.360 |
| 13 | Bianca van der Meer (Neo) | NED | 43.90 | SB | +3.380 |
| 14 | Evy de Wildt (B2) | NED | 44.28 | | +3.760 |
| 15 | Lauren van den Brink (D1) | NED | 44.36 | | +3.840 |
| 16 | Lotte Welboren (C2) | NED | 44.42 | PB | +3.900 |
| Nanette de Boon (D2) | NED | 44.42 | | +3.900 |
| 18 | Milou van Leeuwen (Neo) | NED | 44.50 | SB | +3.980 |
| 19 | Elena Ruijtenberg (C2) | NED | 44.58 | SB | +4.060 |
| 20 | Diede Brandts (B1) | NED | 44.60 | | +4.080 |
| 21 | Marleen Knol (B1) | NED | 44.89 | | +4.370 |
| 22 | Anna.07 Dekker (B1) | NED | 45.17 | PB | +4.650 |
| 23 | Dinte van Es (B2) | NED | 45.22 | SB | +4.700 |
| 24 | Bo Hoogvorst (C2) | NED | 45.39 | PB | +4.870 |
| 25 | Keira Vissenberg (C2) | NED | 46.02 | PB | +5.500 |
| 26 | Floortje Bregman (B2) | NED | 46.14 | | +5.620 |
| 27 | Isabella Talen (B1) | NED | 46.21 | | +5.690 |
| 28 | Amanda van Vugt (C1) | NED | 46.26 | SB | +5.740 |
| 29 | Zoè de Esch (C1) | NED | 46.34 | SB | +5.820 |
| 30 | Evi Blomberg (C1) | NED | 46.40 | | +5.880 |
| 31 | Diede Salomons (C1) | NED | 46.90 | | +6.380 |
| 32 | Maud Heersink (D2) | NED | 47.08 | SB | +6.560 |
| 33 | Veerle Zunnebeld (D2) | NED | 47.10 | | +6.580 |
| 34 | Jildau de Vries (C1) | NED | 47.14 | | +6.620 |
| 35 | Simone Gravesteijn (B1) | NED | 47.17 | SB | +6.650 |
| 36 | Bo Meijer (Neo) | NED | 47.50 | | +6.980 |
| 37 | Anne-Fleur van der Lied (B2) | NED | 47.51 | SB | +6.990 |
| 38 | Ayla Kaldenbach (C1) | NED | 47.61 | | +7.090 |
| 39 | Marthe Schalkwijk (C2) | NED | 48.20 | SB | +7.680 |
| 40 | Imre Blomberg (D1) | NED | 48.58 | SB | +8.060 |
| 41 | Isa van der Weerd (D2) | NED | 49.45 | PB | +8.930 |
| 42 | Ava Zachrisson (D2) | NED | 50.11 | | +9.590 |
| 43 | Pia Rudiger (B1) | NED | 50.27 | | +9.750 |
| 44 | Sanna van der Velde (C1) | NED | 51.04 | SB | +10.520 |
| 45 | Bente van Dasselaar (C1) | NED | 51.42 | | +10.900 |