| 1 | Amber Duizendstraal (Neo) | NED | 39.78 | SB | - |
| 2 | Ju-Lin de Visser (Neo) | NED | 39.91 | SB | +0.130 |
| 3 | Anne Plat (Senior) | NED | 40.12 | SB | +0.340 |
| 4 | Yasmine Bouaziz (Neo) | NED | 40.21 | SB | +0.430 |
| 5 | Amy van der Meer (Neo) | NED | 40.83 | SB | +1.050 |
| 6 | Sietske van der Meer (Neo) | NED | 41.07 | SB | +1.290 |
| 7 | Francisca Gomes Henriques (B2) | POR | 41.58 | NRJ | +1.800 |
| 8 | Hilde Noppert (Neo) | NED | 41.69 | SB | +1.910 |
| 9 | Naomi Kammeraat (B1) | NED | 41.92 | SB | +2.140 |
| 10 | Mette ten Cate (B1) | NED | 42.10 | SB | +2.320 |
| 11 | Tessa Oudman (Senior) | NED | 42.22 | SB | +2.440 |
| 12 | Silke Hijma (Neo) | NED | 42.29 | | +2.510 |
| 13 | Jasmijn Soet (Neo) | NED | 42.69 | SB | +2.910 |
| 14 | Emma Hulshof (Senior) | NED | 42.78 | SB | +3.000 |
| 15 | Anna Marit Sybrandi (Neo) | NED | 42.96 | SB | +3.180 |
| 16 | Karlijn Schellekens (A1) | NED | 43.17 | SB | +3.390 |
| 17 | Sara ter Hart (B2) | NED | 43.56 | SB | +3.780 |
| 18 | Noëmi de Boer (A1) | NED | 43.63 | SB | +3.850 |
| 19 | Marcha Lich (Neo) | NED | 43.84 | SB | +4.060 |
| 20 | Gwen Konter (Neo) | NED | 44.18 | SB | +4.400 |
| 21 | Nynke Tinga (A1) | NED | 44.22 | SB | +4.440 |
| 22 | Nynke Ytje Boonstra (A2) | NED | 44.63 | SB | +4.850 |
| 23 | Amber Koetsier (B1) | NED | 45.32 | SB | +5.540 |
| 24 | Jasmin van der Terp (A1) | NED | 45.60 | SB | +5.820 |
| 25 | Zefina de Bruin (Neo) | NED | 45.65 | SB | +5.870 |
| 26 | Rosa Lieke Smits (A1) | NED | 45.85 | SB | +6.070 |
| 27 | Amina Dillmann (C2) | NED | 46.10 | SB | +6.320 |
| 28 | Jenieke Kuiken (A2) | NED | 46.19 | SB | +6.410 |
| 29 | Maria Procópio Dias (C2) | POR | 46.29 | PB | +6.510 |
| 30 | Anna.07 Dekker (B1) | NED | 46.33 | SB | +6.550 |
| 31 | Lonneke Merkx (B1) | NED | 46.55 | SB | +6.770 |
| 32 | Britt Steg (B1) | NED | 46.74 | SB | +6.960 |
| 33 | Eva Weultjes (B1) | NED | 46.86 | SB | +7.080 |
| 34 | Kim van der Graaf (C2) | NED | 46.94 | SB | +7.160 |
| 35 | Silke Ludema (B2) | NED | 46.96 | SB | +7.180 |
| 36 | Ayla Kaldenbach (C1) | NED | 47.32 | SB | +7.540 |
| 37 | Clara Correia Cruz Pereira (B1) | POR | 48.09 | SB | +8.310 |
| 38 | Else Marie Boersma (C1) | NED | 48.41 | SB | +8.630 |
| 39 | Amber Wietsma (B1) | NED | 49.20 | SB | +9.420 |
| 40 | Ana Rita Andrade de Freitas (B2) | POR | 49.22 | PB | +9.440 |
| 41 | Matilde Sofia Oliveira Ferreira (n/a) | POR | 49.74 | PB | +9.960 |
| 42 | Leonor Almodôvar Ladeira (B1) | POR | 1:03.93 | | +24.150 |
| DNS | Lieke Huizink (B2) | NED | DNS | | - |