| 1 | Marije van der Spek (Neo) | NED | 40.70 | PB | - |
| 2 | Amée Hollanders (Neo) | NED | 41.04 | SB | +0.340 |
| 3 | Tessa Oudman (Senior) | NED | 41.06 | SB | +0.360 |
| 4 | Emma Sylvia de Bock (B2) | USA | 41.51 | SB | +0.810 |
| 5 | Talis Renes (A2) | NED | 41.67 | PB | +0.970 |
| 6 | Rosan Kuip (B2) | NED | 41.93 | PB | +1.230 |
| 7 | Lauren van den Brink (C1) | NED | 41.97 | | +1.270 |
| 8 | Iris Willemsen (Senior) | NED | 42.19 | | +1.490 |
| 9 | Gwen Konter (Neo) | NED | 42.30 | PB | +1.600 |
| 10 | Marleen Knol (A1) | NED | 42.64 | | +1.940 |
| 11 | Marloes Westerhof (M40) | NED | 42.68 | | +1.980 |
| 12 | Silke Ludema (A2) | NED | 43.26 | PB | +2.560 |
| 13 | Feline Scholten (C1) | NED | 43.37 | PB | +2.670 |
| 14 | Maaike Haveman (B2) | NED | 43.41 | | +2.710 |
| 15 | Zoe van der Velden (B1) | NED | 43.55 | PB | +2.850 |
| 16 | Selene Dekker (B1) | NED | 43.62 | | +2.920 |
| 17 | Bente Möller (Neo) | NED | 43.84 | SB | +3.140 |
| 18 | Ava Zachrisson (C2) | SWE | 43.87 | PB | +3.170 |
| 19 | Imre Blomberg (C1) | NED | 43.99 | PB | +3.290 |
| 20 | Zoè de Esch (B1) | NED | 44.06 | PB | +3.360 |
| 21 | Kiara ten Napel (A2) | NED | 44.20 | PB | +3.500 |
| 22 | Annick van der Woude (Senior) | NED | 44.24 | | +3.540 |
| 23 | Wende Linnenbank (C1) | NED | 44.34 | PB | +3.640 |
| 24 | Tirza Blokland (A1) | NED | 44.43 | PB | +3.730 |
| 25 | Emma Fransen (Neo) | NED | 44.51 | PB | +3.810 |
| 26 | Noor Kortschot (B2) | NED | 44.59 | | +3.890 |
| 27 | Fay Barendse (C2) | NED | 45.21 | PB | +4.510 |
| 28 | Madee Hadders (C1) | NED | 45.72 | | +5.020 |
| 29 | Dinte van Es (A2) | NED | 45.80 | SB | +5.100 |
| 30 | Elise Bergé (B1) | BEL | 46.09 | PB | +5.390 |
| 31 | Anne Murk (Neo) | NED | 46.23 | SB | +5.530 |
| 32 | Maren de Bruin (C2) | NED | 46.29 | | +5.590 |
| 33 | Janette de Groot-Bron (M45) | NED | 46.57 | | +5.870 |
| 34 | Lisa Valenteijn (D2) | NED | 47.45 | PB | +6.750 |
| 35 | Jojanneke Scheepstra (M30) | NED | 49.22 | PB | +8.520 |
| 36 | Elise Blokland (B1) | NED | 50.22 | | +9.520 |
| DNF | Noor Klunder (C1) | NED | DNF | | - |
| DNF | Lena de Groot (B2) | NED | DNF | | - |
| DNF | Evi Blomberg (B1) | NED | DNF | | - |