| 1 | Marieke de Vries (Neo) | NED | 40.38 | PB | - |
| 2 | Anne Sofie Knutsen Birkedal (A1) | NOR | 40.41 | | +0.030 |
| 3 | Amy van der Meer (Senior) | NED | 40.57 | | +0.190 |
| 4 | Tessa Oudman (Senior) | NED | 41.10 | SB | +0.720 |
| 5 | Marije van der Spek (Neo) | NED | 41.21 | SB | +0.830 |
| 6 | Lauren van den Brink (C1) | NED | 41.95 | SB | +1.570 |
| 7 | Iris Willemsen (Senior) | NED | 42.17 | SB | +1.790 |
| 8 | Talis Renes (A2) | NED | 42.36 | PB | +1.980 |
| 9 | Marloes Westerhof (M40) | NED | 42.52 | | +2.140 |
| 10 | Lara Dingjan (Neo) | NED | 42.95 | PB | +2.570 |
| 11 | Carlijn te Poele (C2) | NED | 43.07 | PB | +2.690 |
| 12 | Sara van Leeuwen (Neo) | NED | 43.09 | SB | +2.710 |
| 13 | Noa Veerman (C2) | NED | 43.32 | PB | +2.940 |
| 14 | Augustine Trijntje Kaper (C2) | NED | 43.34 | SB | +2.960 |
| 15 | Pauline Tas (B2) | NED | 43.54 | PB | +3.160 |
| 16 | Femke Lindeboom (C2) | NED | 44.23 | PB | +3.850 |
| 17 | Charissa de Mes (B2) | NED | 44.37 | | +3.990 |
| 18 | Veerle Zunnebeld (C2) | NED | 44.40 | | +4.020 |
| 19 | Feline Scholten (C1) | NED | 44.41 | | +4.030 |
| 20 | Marjolein Ooms (B1) | NED | 44.46 | | +4.080 |
| 21 | Zoè de Esch (B1) | NED | 44.53 | PB | +4.150 |
| 22 | Anouk Schepers (C2) | NED | 44.63 | PB | +4.250 |
| 23 | Imre Blomberg (C1) | NED | 44.93 | | +4.550 |
| 24 | Marit Veenhuis (C2) | NED | 45.05 | PB | +4.670 |
| 25 | Dara van Hees (C2) | NED | 45.10 | | +4.720 |
| 26 | Madee Hadders (C1) | NED | 45.24 | | +4.860 |
| 27 | Nynke Lankhaar (A2) | NED | 45.33 | SB | +4.950 |
| 28 | Lonneke Merkx (A1) | NED | 45.37 | | +4.990 |
| 29 | Fay Barendse (C2) | NED | 45.39 | SB | +5.010 |
| 30 | Danique Stout (C1) | NED | 45.50 | PB | +5.120 |
| 31 | Bo Meijer (Neo) | NED | 45.71 | SB | +5.330 |
| 32 | Melanie Ooms (C1) | NED | 45.78 | | +5.400 |
| 33 | Sjuul Te Braake (C2) | NED | 45.85 | SB | +5.470 |
| 34 | Senne Eggenkamp (C2) | NED | 45.89 | PB | +5.510 |
| 35 | Juul van der Vliet (C2) | NED | 45.97 | | +5.590 |
| 36 | Elise Bergé (B1) | BEL | 46.20 | PB | +5.820 |
| 37 | Kersty Heeremans (Neo) | NED | 46.46 | SB | +6.080 |
| 38 | Monique Visser (M35) | NED | 46.68 | SB | +6.300 |
| 39 | Suus Gudema (D2) | NED | 46.87 | PB | +6.490 |
| 40 | Mara Putman (C1) | NED | 47.28 | PB | +6.900 |
| 41 | Mirthe Snoek (C1) | NED | 47.31 | | +6.930 |
| 42 | Fien Heres (C1) | NED | 47.66 | | +7.280 |
| 43 | Femke.11 de Jong (C1) | NED | 47.98 | PB | +7.600 |
| 44 | Jaden Wolthers (C1) | NED | 48.01 | PB | +7.630 |
| 45 | Esra Alkema (D1) | NED | 48.68 | PB | +8.300 |
| 46 | Linn Kolkman (C2) | NED | 49.72 | | +9.340 |
| 47 | Marije Kerkhof (M50) | NED | 50.02 | SB | +9.640 |
| 48 | Linde Stoer (C1) | NED | 58.96 | | +18.580 |
| DNS | Lataesha Narain (Neo) | NED | DNS | | - |