| 1 | Fran Vanhoutte (Neo) | BEL | 39.31 | SB | - |
| 2 | Ju-Lin de Visser (Senior) | TPE | 40.16 | | +0.850 |
| 3 | Amy van der Meer (Senior) | NED | 40.69 | | +1.380 |
| 4 | Jasmijn ter Haar (B2) | NED | 40.86 | SB | +1.550 |
| 5 | Jildou Schaaf (Neo) | NED | 41.02 | SB | +1.710 |
| 6 | Nynke Tinga (Neo) | NED | 41.08 | SB | +1.770 |
| 7 | Fleur Hartveld (C2) | NED | 41.45 | SB | +2.140 |
| 8 | Amée Hollanders (Neo) | NED | 41.46 | SB | +2.150 |
| 9 | Marije van der Spek (Neo) | NED | 41.59 | SB | +2.280 |
| 10 | Emma de Bock (B2) | USA | 41.88 | SB | +2.570 |
| 11 | Marleen Knol (A1) | NED | 42.79 | PB | +3.480 |
| 12 | Iris Willemsen (Senior) | NED | 42.87 | SB | +3.560 |
| 13 | Tessa Oudman (Senior) | NED | 42.94 | | +3.630 |
| 14 | Gwen Konter (Neo) | NED | 43.08 | SB | +3.770 |
| 15 | Noa Petitjean (B2) | NED | 43.16 | SB | +3.850 |
| 16 | Amina Dillmann (B2) | NED | 43.26 | PB | +3.950 |
| 17 | Isabelle Hersbach (Senior) | NED | 43.35 | SB | +4.040 |
| 18 | Josephine Grill (A1) | SWE | 43.36 | PB | +4.050 |
| 19 | Maaike Haveman (B2) | NED | 43.42 | SB | +4.110 |
| 20 | Maureen van Seumeren (B1) | NED | 43.53 | SB | +4.220 |
| 21 | Talis Renes (A2) | NED | 43.63 | SB | +4.320 |
| 22 | Annick van der Woude (Senior) | NED | 44.01 | SB | +4.700 |
| 23 | Anne Breider (B1) | NED | 44.06 | PB | +4.750 |
| 24 | Evi Blomberg (B1) | NED | 44.09 | SB | +4.780 |
| 25 | Diede Brandts (A1) | NED | 44.45 | | +5.140 |
| 26 | Ava Zachrisson (C2) | SWE | 44.49 | PB | +5.180 |
| 27 | Irene Hörnschemeyer (A1) | GER | 44.50 | SB | +5.190 |
| 28 | Silke Ludema (A2) | NED | 44.86 | SB | +5.550 |
| 29 | Imre Blomberg (C1) | NED | 44.91 | SB | +5.600 |
| 30 | Fiore Koutstaal (B1) | NED | 45.06 | SB | +5.750 |
| 31 | Youssra Pals (B2) | NED | 45.23 | SB | +5.920 |
| 32 | Yfke Vreeken (C1) | NED | 45.45 | PB | +6.140 |
| 33 | Hiba Pals (C2) | NED | 45.63 | PB | +6.320 |
| 34 | Zoè de Esch (B1) | NED | 45.72 | SB | +6.410 |
| 35 | Noor Kortschot (B2) | NED | 46.02 | SB | +6.710 |
| 36 | Femke Lindeboom (C2) | NED | 46.10 | PB | +6.790 |
| 37 | Josephine Dekkers (B2) | NED | 46.16 | PB | +6.850 |
| 38 | Nina Heethaar (B1) | NED | 46.41 | SB | +7.100 |
| 39 | Yasmine Pals (B2) | NED | 46.65 | PB | +7.340 |
| 40 | Maartje Onderdelinden (B2) | NED | 46.75 | SB | +7.440 |
| 41 | Naomi Segers (C1) | NED | 47.26 | | +7.950 |
| 42 | Danique Stout (C1) | NED | 47.33 | PB | +8.020 |
| 43 | Eline Mulder (A2) | BEL | 47.51 | SB | +8.200 |
| 44 | Antje Ypma (C2) | NED | 47.57 | SB | +8.260 |
| 45 | Maud Heersink (C2) | NED | 48.18 | SB | +8.870 |
| 46 | Ilse Kolmer (B1) | NED | 49.21 | SB | +9.900 |
| 47 | Julia Onderdelinden (C2) | NED | 50.04 | SB | +10.730 |