| 1 | Fleur Smits (B1) | NED | 43.73 | | - |
| 2 | Femke Assink (Neo) | NED | 45.46 | | +1.730 |
| 3 | Noëmi de Boer (B2) | NED | 45.47 | | +1.740 |
| 4 | Charley Boekema (E2) | NED | 46.89 | | +3.160 |
| 5 | Kalista Lamers (B1) | NED | 47.03 | SB | +3.300 |
| 6 | Lenneke Groot Koerkamp (B2) | NED | 47.76 | | +4.030 |
| 7 | Chantal Rietman (M45) | NED | 47.92 | | +4.190 |
| 8 | Elena Ruijtenberg (C1) | NED | 48.11 | | +4.380 |
| 9 | Iris Rietman (C2) | NED | 48.14 | | +4.410 |
| 10 | Marthe Schalkwijk (C1) | NED | 48.72 | SB | +4.990 |
| 11 | Isis van Til (C2) | NED | 48.86 | PB | +5.130 |
| 12 | Benthe Kamerling (D1) | NED | 48.98 | | +5.250 |
| 13 | Lauren van den Brink (E2) | NED | 49.18 | | +5.450 |
| 14 | Liz Wolters (D1) | NED | 49.21 | | +5.480 |
| 15 | Norah van Elst (E2) | NED | 50.67 | | +6.940 |
| Morgan Defreese (E2) | NED | 50.67 | | +6.940 |
| 17 | Lisa Otten (C1) | NED | 51.69 | | +7.960 |
| 18 | Monique de Groot (D2) | NED | 51.79 | | +8.060 |
| 19 | Isa van der Weerd (D1) | NED | 51.88 | | +8.150 |
| 20 | Noor Klunder (E2) | NED | 52.56 | SB | +8.830 |
| 21 | Liss Phielix (C1) | NED | 52.91 | | +9.180 |
| 22 | Bente van Dasselaar (D2) | NED | 52.98 | | +9.250 |
| 23 | Marit Noordman (C2) | NED | 53.57 | | +9.840 |
| 24 | Sanna van der Velde (D2) | NED | 54.00 | | +10.270 |
| 25 | Silke van den Bos (D2) | NED | 55.26 | | +11.530 |
| 26 | Mireille van der Weerd (C1) | NED | 56.10 | | +12.370 |
| 27 | Emma van der Velde (E2) | NED | 56.83 | | +13.100 |
| 28 | Femke Lindeboom (D1) | NED | 57.23 | | +13.500 |
| 29 | Lauren Wolters (E1) | NED | 57.55 | PB | +13.820 |
| 30 | Famke Aukema (D2) | NED | 57.78 | | +14.050 |
| 31 | Elin Mak (F2) | NED | 58.93 | SB | +15.200 |
| 32 | Eline Tuitert (E2) | NED | 59.62 | SB | +15.890 |
| 33 | Tess de Waal (E2) | NED | 1:02.33 | | +18.600 |
| 34 | Anne van Duinen (E2) | NED | 1:02.63 | SB | +18.900 |
| 35 | Kim Versteeg (D1) | NED | 1:02.75 | | +19.020 |
| 36 | Thea de Groot (B1) | NED | 1:04.03 | | +20.300 |
| 37 | Jitske van de Hel (C1) | NED | 1:04.54 | | +20.810 |
| 38 | Carlijn van Dongen (C1) | NED | 1:04.95 | | +21.220 |
| 39 | Karin Mulder (Senior) | NED | 1:05.34 | SB | +21.610 |
| DQ | Jill Krikken (E1) | NED | DQ | | - |