| 1 | Linda Klein (B2) | NED | 44.83 | | - |
| 2 | Meeke Rijnen (B1) | NED | 45.19 | | +0.360 |
| 3 | Martje Wisse (Neo) | NED | 45.20 | SB | +0.370 |
| 4 | Monica Ravenstijn (B1) | NED | 46.18 | | +1.350 |
| 5 | Esther Arts (C2) | NED | 46.31 | | +1.480 |
| 6 | Mirjam Rottier (B1) | NED | 47.89 | | +3.060 |
| 7 | Christine van Seters (B1) | NED | 48.19 | | +3.360 |
| 8 | Noor van der Ster (M50) | NED | 48.25 | | +3.420 |
| 9 | Leila Sophie Otten (B1) | NED | 48.34 | | +3.510 |
| 10 | Lisa van der Zee (C1) | NED | 48.67 | | +3.840 |
| 11 | Clarissa van Maaren (C2) | NED | 48.69 | | +3.860 |
| 12 | Eline Muskens (C1) | NED | 48.85 | | +4.020 |
| 13 | Annemarie Pasveer (B1) | NED | 49.68 | | +4.850 |
| 14 | Claudia van Heel (M35) | NED | 49.86 | | +5.030 |
| 15 | Linsey Kaïn (A2) | NED | 50.84 | | +6.010 |
| 16 | Lise Visser (C1) | NED | 50.86 | | +6.030 |
| 17 | Janneke Elzinga (C2) | NED | 52.38 | | +7.550 |
| 18 | Aniek Jongerius (Neo) | NED | 52.41 | | +7.580 |
| 19 | Wendie op de Beek (Senior) | NED | 52.54 | | +7.710 |
| 20 | Linda Holster (M30) | NED | 53.14 | SB | +8.310 |
| 21 | Daphne Guerra (D1) | NED | 53.37 | | +8.540 |
| 22 | Jolien Jongerius (Neo) | NED | 53.37 | | +8.540 |
| 23 | Karin Dekker (B1) | NED | 53.83 | | +9.000 |
| 24 | Imke van der Steen (Neo) | NED | 53.95 | | +9.120 |
| 25 | Elsemieke van Maaren (D1) | NED | 54.02 | | +9.190 |
| 26 | Imke de Klerk (C2) | NED | 54.17 | | +9.340 |
| 27 | Ineke Bijl (Senior) | NED | 54.60 | | +9.770 |
| 28 | Laura de Nijs (C2) | NED | 55.05 | | +10.220 |
| 29 | Tialda Gerritsma (M40) | NED | 55.09 | | +10.260 |
| 30 | Rian Buitendijk (M40) | NED | 56.78 | | +11.950 |
| 31 | Vera Hartman (E2) | NED | 57.16 | | +12.330 |
| 32 | Heidi den Braber (D2) | NED | 57.47 | | +12.640 |
| 33 | Sifra Blok (D2) | NED | 58.73 | SB | +13.900 |
| 34 | Fleur Boogmans (D2) | NED | 1:01.52 | | +16.690 |
| 35 | Esmay Jansen (D2) | NED | 1:02.43 | SB | +17.600 |
| 36 | Renee Leijten (D2) | NED | 1:02.75 | | +17.920 |
| 37 | Lisanne Knuistingh Neven (E2) | NED | 1:03.47 | SB | +18.640 |
| 38 | Marieke Knuistingh Neven (D2) | NED | 1:04.01 | SB | +19.180 |
| 39 | Anne de Weerdt (D2) | NED | 1:04.29 | SB | +19.460 |
| 40 | Jessica van Ginneken (D1) | NED | 1:05.63 | | +20.800 |
| 41 | Kaylee Hendrickx (D2) | NED | 1:06.12 | | +21.290 |
| DNS | Laury van Lieshout (D1) | NED | DNS | | - |