| 1 | Fabienne Maarse (A1) | NED | 44.06 | | - |
| 2 | Danique van Rooden (A2) | NED | 44.58 | SB | +0.520 |
| 3 | Anouk Könst (Neo) | NED | 44.68 | | +0.620 |
| 4 | Zoe van der Velden (B1) | NED | 44.79 | PB | +0.730 |
| 5 | Maud Kempenaar (A1) | NED | 44.98 | SB | +0.920 |
| 6 | Bente Adema (B2) | NED | 45.36 | | +1.300 |
| 7 | Ilse van der Velden (B1) | NED | 45.61 | SB | +1.550 |
| 8 | Tessel Huizinga (B1) | NED | 46.07 | SB | +2.010 |
| 9 | Milou Butter (Senior) | NED | 46.17 | SB | +2.110 |
| 10 | Evy van Duijn (A1) | NED | 46.66 | SB | +2.600 |
| 11 | Lianne van Assema (B2) | NED | 46.86 | SB | +2.800 |
| 12 | Kristel van Abswoude (Senior) | NED | 47.01 | SB | +2.950 |
| 13 | Kersty Heeremans (Neo) | NED | 47.04 | SB | +2.980 |
| 14 | Anouk Rodenburg (A1) | NED | 48.18 | SB | +4.120 |
| 15 | Britt van Wijk (A1) | NED | 48.25 | SB | +4.190 |
| 16 | Aranka Keur (M40) | NED | 48.91 | SB | +4.850 |
| 17 | Lois Elstgeest (B1) | NED | 49.09 | SB | +5.030 |
| 18 | Charline Coates-Kwadrin (A2) | GBR | 49.85 | SB | +5.790 |
| Emma Hartveld (Neo) | NED | 49.85 | SB | +5.790 |
| 20 | Eline Schmutzer (B2) | NED | 50.18 | SB | +6.120 |
| 21 | Noa Bierens (A1) | NED | 50.49 | SB | +6.430 |
| 22 | Anne Lubbers (A1) | NED | 50.78 | SB | +6.720 |
| 23 | Lis uit den Boogaard (B1) | NED | 50.85 | SB | +6.790 |
| 24 | Sylvie van den Heuvel (Neo) | NED | 51.00 | SB | +6.940 |
| 25 | Britt van Schie (B1) | NED | 51.04 | SB | +6.980 |
| 26 | Willemijn Schouwenaar (A2) | NED | 51.12 | | +7.060 |
| 27 | Wieteke Huiberts (Neo) | NED | 52.86 | SB | +8.800 |
| 28 | Lisanne Hartveld (B2) | NED | 53.61 | SB | +9.550 |
| 29 | Pien Lodder (Neo) | NED | 53.73 | SB | +9.670 |
| 30 | Naomi Bakker (C2) | NED | 54.71 | PB | +10.650 |
| 31 | Zoe Verhagen (B2) | NED | 1:00.25 | SB | +16.190 |
| 32 | Kelly Tromp (Senior) | NED | 1:01.07 | SB | +17.010 |
| 33 | Meggy van Dijk-Brolsma (M50) | NED | 1:05.23 | | +21.170 |
| WDR | Stefanie Koot (A1) | NED | WDR | | - |