| 1 | Janine Smit (Senior) | NED | 39.34 | TR | - |
| 2 | Mayon Kuipers (Senior) | NED | 39.56 | | +0.220 |
| Letitia de Jong (Neo) | NED | 39.56 | | +0.220 |
| 4 | Moniek Klijnstra (Neo) | NED | 39.70 | | +0.360 |
| 5 | Rosa Pater (Senior) | NED | 39.76 | | +0.420 |
| 6 | Saori Toi (Neo) | JPN | 39.87 | | +0.530 |
| 7 | Annouk van der Weijden (Senior) | NED | 39.95 | | +0.610 |
| 8 | Bente van den Berge (Neo) | NED | 40.06 | | +0.720 |
| 9 | Roxanne van Hemert (Senior) | NED | 40.29 | | +0.950 |
| 10 | Linda de Vries (Senior) | NED | 40.55 | | +1.210 |
| 11 | Emma van Rijn (Neo) | NED | 40.72 | | +1.380 |
| 12 | Leeyen Harteveld (Neo) | NED | 40.85 | | +1.510 |
| 13 | Yvonne Nauta (Senior) | NED | 41.03 | | +1.690 |
| 14 | Maud van der Meer (Neo) | NED | 41.11 | SB | +1.770 |
| 15 | Melinda Groot (Neo) | NED | 41.25 | SB | +1.910 |
| 16 | Sanne van der Schaar (Neo) | NED | 41.27 | | +1.930 |
| 17 | Annemarie Boer (Neo) | NED | 41.35 | | +2.010 |
| 18 | Marleen de Kroon (Neo) | NED | 41.48 | PB | +2.140 |
| 19 | Tessa van der Neut (Neo) | NED | 41.58 | | +2.240 |
| 20 | Reina Anema (Neo) | NED | 41.60 | | +2.260 |
| 21 | Julia Berentschot (Neo) | NED | 41.62 | | +2.280 |
| 22 | Steffi Wubben (Neo) | NED | 41.70 | | +2.360 |
| 23 | Manouk van Tol (Senior) | NED | 41.71 | | +2.370 |
| Natasja Roest (Neo) | NED | 41.71 | SB | +2.370 |
| 25 | Maike Brinksma (Neo) | NED | 42.04 | | +2.700 |
| 26 | Mignon Goeree (Neo) | NED | 42.16 | | +2.820 |
| 27 | Eva-Sterre Bording (Neo) | NED | 42.23 | | +2.890 |
| 28 | Kelly Eijsink (Neo) | NED | 42.67 | | +3.330 |
| 29 | Marjanne Timmer (Neo) | NED | 43.69 | | +4.350 |
| DQ | Vera Otten (Neo) | NED | DQ | | - |