| 1 | Iris Olde Wolsink (Senior) | NED | 48.08 | | - |
| 2 | Iris Bosch (Senior) | NED | 48.09 | SB | +0.010 |
| 3 | Aniek Hesselink (A2) | NED | 48.24 | | +0.160 |
| 4 | Emma Wolters (A2) | NED | 48.92 | | +0.840 |
| 5 | Eloise van der Sligte (C1) | NED | 49.32 | | +1.240 |
| 6 | Elisa Stenvers (C2) | NED | 49.80 | | +1.720 |
| 7 | Jildou van Felius (A1) | NED | 50.15 | | +2.070 |
| 8 | Yuna Broeze (C1) | NED | 51.63 | | +3.550 |
| 9 | Esmee Hesselink (D1) | NED | 54.40 | SB | +6.320 |
| 10 | Fieke Kramer (D1) | NED | 1:01.37 | PB | +13.290 |
| 11 | Mar Schouten (E2) | NED | 1:08.73 | | +20.650 |
| 12 | Babet Lubbers (E1) | NED | 1:09.51 | SB | +21.430 |
| 13 | Imke Mogezomp (D1) | NED | 1:11.82 | SB | +23.740 |
| 14 | Sarah Kalsbeek (E1) | NED | 1:12.81 | SB | +24.730 |
| 15 | Meijs Dikken (E1) | NED | 1:14.63 | SB | +26.550 |
| 16 | Jorne Kramer (E1) | NED | 1:15.15 | | +27.070 |
| 17 | Lauren Stopel (E1) | NED | 1:16.69 | PB | +28.610 |
| 18 | Christel Scheiberlich (E2) | NED | 1:19.47 | SB | +31.390 |
| 19 | Elisa Lelifeld (E1) | NED | 1:23.21 | SB | +35.130 |
| 20 | Annelien de Vries (Senior) | NED | 2:31.96 | SB | +1.43.880 |
| DNF | Benthe Blaak (E1) | NED | DNF | | - |