| 1 | Ellen Hazelaar (Neo) | NED | 42.13 | | - |
| 2 | Moniek Kleinsman (Senior) | NED | 42.55 | | +0.420 |
| 3 | Britta Herlitz (Neo) | GER | 42.72 | | +0.590 |
| 4 | Cindy Vergeer (Neo) | NED | 42.81 | | +0.680 |
| 5 | Carlijn Achtereekte (Neo) | NED | 43.43 | | +1.300 |
| 6 | Marlies ter Bekke (Neo) | NED | 43.57 | | +1.440 |
| 7 | Pien Keulstra (B1) | NED | 44.06 | | +1.930 |
| 8 | Chantal van Marle (Senior) | NED | 44.11 | | +1.980 |
| 9 | Patricia Wieberdink (A2) | NED | 44.39 | | +2.260 |
| 10 | Paulien Westerhof (A2) | NED | 44.43 | | +2.300 |
| 11 | Jinthe Roelofs (B1) | NED | 44.97 | | +2.840 |
| 12 | Monica Ravenstijn (B2) | NED | 44.98 | | +2.850 |
| 13 | Dominique Lommers (C1) | NED | 45.03 | | +2.900 |
| 14 | Matia Kosters (A2) | NED | 45.06 | | +2.930 |
| 15 | Renske Winters (M30) | NED | 45.08 | | +2.950 |
| 16 | Ingeborg Betz (B2) | NED | 45.39 | | +3.260 |
| 17 | Anneke ten Hove (Neo) | NED | 45.48 | | +3.350 |
| 18 | Merle Jorink (B2) | NED | 45.60 | | +3.470 |
| 19 | Jekaterina Kozelkova (C2) | RUS | 46.48 | | +4.350 |
| 20 | Eline Vollenbroek (Neo) | NED | 46.68 | | +4.550 |
| 21 | Madelien Jansen (B2) | NED | 46.89 | | +4.760 |
| 22 | Annet Pit (B2) | NED | 46.96 | | +4.830 |
| 23 | Ksenija Zjavoronkova (D2) | RUS | 47.21 | | +5.080 |
| 24 | Kristina Tsjernakova (C1) | RUS | 47.49 | | +5.360 |
| 25 | Anastasija Zdravkova (C1) | RUS | 48.44 | | +6.310 |
| 26 | Dorris te Rietmole (C2) | NED | 48.63 | | +6.500 |
| 27 | Lianne Versluis (B1) | NED | 49.15 | | +7.020 |
| 28 | Milena Syrnikova (D1) | RUS | 51.74 | | +9.610 |
| 29 | Regina Jaminova (D2) | RUS | 1:01.62 | | +19.490 |