| 1 | Brittany Bowe (Senior) | USA | 38.54 | | - |
| 2 | Sarah Warren (Senior) | USA | 39.45 | | +0.910 |
| 3 | Chrysta Rands-Evans (Senior) | USA | 39.64 | | +1.100 |
| 4 | Greta Myers (A2) | USA | 40.37 | | +1.830 |
| 5 | Mia Kilburg-Manganello (Senior) | USA | 40.67 | | +2.130 |
| 6 | Anna Quinn (Neo) | USA | 40.68 | | +2.140 |
| 7 | Lindsey Woodbury (Neo) | USA | 42.25 | | +3.710 |
| 8 | Giorgia Birkeland (Neo) | USA | 42.53 | | +3.990 |
| 9 | Shruti Kotwal (M30) | IND | 42.99 | | +4.450 |
| 10 | Natalia Valencia (Senior) | COL | 43.04 | | +4.500 |
| 11 | Ilsa Melin Sandvig Shobe (Senior) | USA | 43.29 | | +4.750 |
| 12 | Isabela Vargas Rodriguez (B1) | COL | 44.44 | | +5.900 |
| 13 | Ella Teeples (C1) | USA | 45.17 | | +6.630 |
| 14 | Karen Lizeth Siatama Valencia (B2) | COL | 46.55 | | +8.010 |
| 15 | Kaitlyn Lee (C1) | USA | 48.03 | | +9.490 |
| 16 | Avery Hayes (C1) | USA | 49.12 | | +10.580 |
| 17 | Audrey Han (C2) | USA | 49.22 | | +10.680 |
| 18 | Scarelette Gramlich Thatcher (D1) | USA | 51.58 | | +13.040 |
| 19 | Juliette Song (D1) | USA | 51.96 | SB | +13.420 |
| 20 | Nihan Tatli (n/a) | USA | 1:04.65 | | +26.110 |
| 21 | Atira Meacham (F2) | USA | 1:06.76 | SB | +28.220 |