| 1 | Kimi Goetz (Senior) | USA | 37.84 | | - |
| 2 | Erin Jackson (Senior) | USA | 38.19 | | +0.350 |
| 3 | Yu-Ting Huang (Senior) | TPE | 38.83 | | +0.990 |
| 4 | Maria Victoria Rodriguez (Senior) | ARG | 39.40 | | +1.560 |
| 5 | McKenzie Browne (Senior) | USA | 39.93 | | +2.090 |
| 6 | Chrysta Rands (Senior) | USA | 40.07 | | +2.230 |
| 7 | Mia Kilburg-Manganello (Senior) | USA | 40.13 | SB | +2.290 |
| 8 | Paige Schwartzburg (Senior) | USA | 40.18 | SB | +2.340 |
| 9 | Altan-Ochir Zul (Senior) | MGL | 41.58 | | +3.740 |
| 10 | Laura Michelle Rodriguez (B1) | COL | 41.59 | NRJ, NR | +3.750 |
| 11 | Dessie Weigel (Senior) | USA | 41.64 | SB | +3.800 |
| 12 | Anna Quinn (Neo) | USA | 41.74 | | +3.900 |
| 13 | Lindsey Woodbury (A2) | USA | 41.95 | | +4.110 |
| 14 | Jamie Jurak (M30) | USA | 42.04 | SB | +4.200 |
| 15 | Kristiine Kalev (Neo) | EST | 42.16 | | +4.320 |
| 16 | Jamie Lindquist-Nielson (Neo) | USA | 42.70 | SB | +4.860 |
| 17 | Shruti Kotwal (M30) | IND | 43.07 | | +5.230 |
| 18 | Ilsa Melin Sandvig Shobe (Neo) | USA | 43.14 | | +5.300 |
| 19 | Cynthia Jennings (Senior) | USA | 43.25 | | +5.410 |
| 20 | Laura Gomez Quintero (Senior) | COL | 43.31 | SB | +5.470 |
| 21 | Mary Brophy (M45) | USA | 43.35 | SB | +5.510 |
| 22 | Larissa Paes (Senior) | BRA | 43.42 | | +5.580 |
| 23 | Marley Soldan (C2) | USA | 44.44 | | +6.600 |
| 24 | Isabela Vargas Rodriguez (C2) | COL | 44.90 | | +7.060 |
| 25 | Libby Williams (B2) | USA | 45.69 | | +7.850 |
| 26 | Leah Choi (C1) | USA | 47.13 | SB | +9.290 |
| 27 | Ella Teeples (D2) | USA | 48.19 | | +10.350 |
| 28 | Finnley D'Card (D2) | USA | 48.60 | SB | +10.760 |
| 29 | Avery Hayes (D2) | USA | 53.45 | | +15.610 |
| 30 | Scarelette Gramlich Thatcher (E2) | USA | 55.13 | | +17.290 |
| 31 | Kaitlyn Lee (D2) | USA | 56.52 | SB | +18.680 |
| 32 | Juliette Song (E2) | USA | 58.30 | SB | +20.460 |
| DNS | Valentina Espinosa (Neo) | ARG | DNS | | - |
| DQ | Sarah Warren (Senior) | USA | DQ | | - |