| 1 | Béatrice Lamarche (Senior) | CAN | 38.80 | SB | - |
| 2 | Rose Laliberté-Roy (Senior) | CAN | 39.69 | SB | +0.890 |
| 3 | Camille Tremblay (Neo) | CAN | 41.06 | | +2.260 |
| 4 | Molly Flynn (B2) | CAN | 41.21 | SB | +2.410 |
| 5 | Cendrine Levesque (Senior) | CAN | 41.34 | SB | +2.540 |
| Léa Tessier (Senior) | CAN | 41.34 | | +2.540 |
| 7 | Cloé Jelonek (Neo) | CAN | 41.47 | SB | +2.670 |
| 8 | Frédérike Béland (Neo) | CAN | 42.08 | SB | +3.280 |
| 9 | Aby-Léonie Ward (B1) | CAN | 42.31 | SB | +3.510 |
| 10 | Coralie Boulais (B1) | CAN | 42.38 | PB | +3.580 |
| 11 | Érica Durand (A1) | CAN | 42.48 | PB | +3.680 |
| 12 | Marie Ferlatte (Neo) | CAN | 42.91 | SB | +4.110 |
| 13 | Léanne Sirois (A2) | CAN | 43.08 | SB | +4.280 |
| 14 | Maude Perron (B1) | CAN | 43.23 | PB | +4.430 |
| 15 | Oriana Sofia Cardenas Loaiza (A1) | CAN | 43.27 | | +4.470 |
| 16 | Eva Marek (B1) | CAN | 43.43 | PB | +4.630 |
| 17 | Lily-Pier Guérin (Neo) | CAN | 43.84 | SB | +5.040 |
| 18 | Magalie Beaulieu (C2) | CAN | 44.04 | PB | +5.240 |
| 19 | Laurence Charron (B2) | CAN | 44.53 | SB | +5.730 |
| 20 | Raphaëlle Auger (B1) | CAN | 44.87 | PB | +6.070 |
| 21 | Frédérique-Olivia Laporte (B2) | CAN | 44.91 | PB | +6.110 |
| 22 | Zitlalli Gravel (n/a) | CAN | 45.02 | SB | +6.220 |
| 23 | Marianne Demers (B1) | CAN | 45.38 | SB | +6.580 |
| 24 | Émilie Mercier (B1) | CAN | 45.50 | SB | +6.700 |
| 25 | Charlyne Deblois (C2) | CAN | 45.58 | PB | +6.780 |
| 26 | Charlotte Babin (B2) | CAN | 45.83 | | +7.030 |
| 27 | Laurie Paquette (C2) | CAN | 46.01 | | +7.210 |
| 28 | Violet Velicevic (B1) | CAN | 46.02 | PB | +7.220 |
| 29 | Marianne Lavoie (C2) | CAN | 46.11 | PB | +7.310 |
| 30 | Laurie Crête (B1) | CAN | 46.12 | PB | +7.320 |
| 31 | Cassandre Desrosiers (B2) | CAN | 46.55 | PB | +7.750 |
| 32 | Gabrielle Boulianne (C2) | CAN | 46.96 | | +8.160 |
| 33 | Myah Payne Landon (Neo) | CAN | 47.29 | SB | +8.490 |
| 34 | Isla Mason (B1) | CAN | 47.57 | | +8.770 |
| 35 | Maïté Cadieux (C2) | CAN | 47.96 | SB | +9.160 |
| 36 | Kallianna Lantagne (B1) | CAN | 48.34 | SB | +9.540 |
| 37 | Clara Smits-Talving (C2) | CAN | 48.37 | PB | +9.570 |
| 38 | Charlie Tremblay (C2) | CAN | 48.40 | PB | +9.600 |
| 39 | Anabel Tanguay (C2) | CAN | 48.74 | PB | +9.940 |
| 40 | Mélivia Bouchard (C2) | CAN | 49.06 | SB | +10.260 |
| 41 | Sylvie Heung (A1) | CAN | 49.58 | | +10.780 |
| 42 | Sara Merheb (C2) | CAN | 50.32 | SB | +11.520 |
| 43 | Abby Karcher (B2) | CAN | 50.58 | | +11.780 |
| 44 | Jade Pelletier (B2) | CAN | 50.70 | SB | +11.900 |
| 45 | Maelie Laplante (B1) | CAN | 50.94 | PB | +12.140 |
| 46 | Mirthe De Haan (B2) | NED | 51.13 | SB | +12.330 |
| 47 | Nelly Faucher (B1) | CAN | 52.45 | SB | +13.650 |
| 48 | Juliana Daigle (B2) | CAN | 53.17 | SB | +14.370 |
| 49 | Daphné Mimeault (B1) | CAN | 58.98 | SB | +20.180 |
| DQ | Caitlin Besso (B1) | CAN | DQ | | - |