| 1 | Talis Renes (A1) | NED | 44.60 | | - |
| 2 | Liotte Smits (Senior) | NED | 44.97 | | +0.370 |
| 3 | Janine Timmer (Neo) | NED | 45.51 | SB | +0.910 |
| 4 | Josephine Dekkers (B1) | NED | 46.35 | SB | +1.750 |
| 5 | Anniek Heethaar (B2) | NED | 46.52 | | +1.920 |
| 6 | Norah Smits (Neo) | NED | 46.97 | | +2.370 |
| 7 | Leoniek Anema (Senior) | NED | 47.05 | | +2.450 |
| 8 | Julia Onderdelinden (C1) | NED | 50.59 | | +5.990 |
| 9 | Madelief Faneyte (B2) | NED | 52.72 | | +8.120 |
| 10 | Samantha Heerschop (Senior) | NED | 56.60 | PB | +12.000 |
| 11 | Karin Hiemstra (Senior) | NED | 59.32 | SB | +14.720 |
| 12 | Nova Ruitenbeek (D1) | NED | 59.84 | | +15.240 |
| 13 | Marjolein Bos (n/a) | NED | 59.93 | PB | +15.330 |
| 14 | Monique Hiemstra (Senior) | NED | 1:00.29 | SB | +15.690 |
| 15 | Linde van Logtenstein (E2) | NED | 1:02.62 | | +18.020 |
| 16 | Louise Immerzeel (Neo) | NED | 1:02.82 | SB | +18.220 |
| 17 | Janneke Boersma-Jentink (n/a) | NED | 1:04.93 | PB | +20.330 |
| 18 | Froukje Onderdelinden (n/a) | NED | 1:05.96 | PB | +21.360 |
| 19 | Josje van der Neut (D2) | NED | 1:10.75 | | +26.150 |
| 20 | Marianne van Immerzeel-Smit (M55) | NED | 1:15.08 | PB | +30.480 |