| 1 | Magalie Beaulieu (C1) | CAN | 46.21 | | - |
| 2 | Charlyne Deblois (C1) | CAN | 46.37 | SB | +0.152 |
| 3 | Flavie Desjardins (D2) | CAN | 46.66 | SB | +0.448 |
| 4 | Anamé Fleury (D2) | CAN | 47.34 | | +1.122 |
| 5 | Alice Parent (D2) | CAN | 47.73 | | +1.514 |
| 6 | Marianne Lavoie (C1) | CAN | 47.92 | | +1.709 |
| 7 | Maïté Cadieux (C1) | CAN | 48.80 | | +2.582 |
| 8 | Sara Merheb (C1) | CAN | 48.88 | PB | +2.666 |
| 9 | Eliane Gaudreault (n/a) | CAN | 48.96 | | +2.741 |
| 10 | Anabel Tanguay (C1) | CAN | 49.08 | SB | +2.861 |
| 11 | Billie Albisser (D2) | CAN | 49.33 | SB | +3.119 |
| 12 | Chloé Lemieux (D2) | CAN | 49.57 | SB | +3.358 |
| 13 | Charlie Tremblay (C1) | CAN | 49.70 | | +3.486 |
| 14 | Mélivia Bouchard (C1) | CAN | 49.93 | | +3.714 |
| 15 | Maika Roy (D2) | CAN | 50.61 | PB | +4.400 |
| 16 | Anne Robichaud (D1) | CAN | 50.87 | PB | +4.660 |
| 17 | Amelia Velez (n/a) | CAN | 51.07 | PB | +4.855 |
| 18 | Anne-Marie Blanchette (D2) | CAN | 51.54 | SB | +5.326 |
| 19 | Naelie Colin (D1) | CAN | 51.62 | SB | +5.404 |
| 20 | Magalie Faucher (D2) | CAN | 51.81 | SB | +5.593 |
| 21 | Eliane Blanchette (D2) | CAN | 52.10 | PB | +5.890 |
| 22 | Maude Roux (C1) | CAN | 52.72 | | +6.510 |
| 23 | Clarisse Larouche (D1) | CAN | 52.81 | SB | +6.598 |
| 24 | Loralie Fallon (D1) | CAN | 52.99 | | +6.776 |
| 25 | Laurence Poirier (D1) | CAN | 53.64 | | +7.429 |
| 26 | Mélia Bolduc (D1) | CAN | 54.35 | SB | +8.134 |
| 27 | Xunzhi Zhang (D1) | CAN | 54.44 | SB | +8.222 |
| 28 | Amandine Ayotte (D2) | CAN | 54.46 | SB | +8.242 |
| 29 | Lou Lepoutre (E2) | CAN | 54.47 | SB | +8.260 |
| 30 | Rosalie St-Hilaire (E2) | CAN | 54.57 | SB | +8.356 |
| 31 | Eloise Beauvais (D1) | CAN | 54.74 | | +8.526 |
| 32 | Delphine Dénommée (n/a) | CAN | 54.90 | PB | +8.688 |
| 33 | Megan L'Espérance (C1) | CAN | 55.10 | | +8.889 |
| 34 | Maude Laquerre (n/a) | CAN | 56.27 | | +10.056 |
| 35 | Rosalie Paquet (D1) | CAN | 56.50 | | +10.284 |
| 36 | Jayden Guerin-girard (n/a) | CAN | 56.59 | PB | +10.376 |
| 37 | Elsa.13 Lebel (D1) | CAN | 56.62 | | +10.402 |
| 38 | Lily Hellenthal-Stewart (E2) | CAN | 57.25 | SB | +11.038 |
| 39 | Amara Gaudreault Chafiqi (E2) | CAN | 58.57 | SB | +12.357 |
| 40 | Lara Amara Martinez Genel (D1) | CAN | 59.37 | SB | +13.160 |
| 41 | Noemie Francoeur-paquette (n/a) | CAN | 59.38 | PB | +13.163 |
| 42 | Ève Robitaille (D2) | CAN | 59.53 | SB | +13.316 |
| 43 | Clara McKendry (E2) | USA | 1:00.64 | | +14.430 |
| 44 | Gabrielle Marchand (E2) | CAN | 1:00.65 | SB | +14.432 |
| 45 | Naomi L'Espérance (D1) | CAN | 1:01.03 | PB | +14.820 |
| 46 | Kaylia Marois (E2) | CAN | 1:01.95 | SB | +15.734 |
| 47 | Pien Bruyninckx (E2) | CAN | 1:05.25 | | +19.037 |