| 1 | Shannon Rempel (Senior) | CAN | 38.80 | | - |
| 2 | Allie Thunstrom (Senior) | USA | 39.00 | PB | +0.200 |
| 3 | Jerica Tandiman (Neo) | USA | 39.27 | | +0.470 |
| 4 | Rebekah Bradford-Plath (M35) | USA | 39.45 | | +0.650 |
| 5 | Noémie Fiset (Neo) | CAN | 39.48 | | +0.680 |
| 6 | Courtney Shmyr (Senior) | CAN | 39.53 | | +0.730 |
| 7 | Maria Victoria Rodriguez (Senior) | ARG | 39.81 | NR | +1.010 |
| 8 | Kerri Morrison (Senior) | CAN | 39.82 | SB | +1.020 |
| 9 | Maddison Pearman (Neo) | CAN | 39.85 | | +1.050 |
| 10 | Mihaela Hogas (A2) | ROU | 39.99 | NRJ | +1.190 |
| 11 | Franchesca Pasquarella (Neo) | USA | 40.07 | | +1.270 |
| 12 | Brianna Bocox (Neo) | USA | 40.08 | | +1.280 |
| 13 | Brooklyn McDougall (A2) | CAN | 40.25 | | +1.450 |
| 14 | Chrysta Rands (Neo) | USA | 40.28 | SB | +1.480 |
| 15 | Yauheniya Varabyova (Neo) | BLR | 40.30 | SB | +1.500 |
| 16 | Keira Poelzer (Senior) | CAN | 40.48 | | +1.680 |
| 17 | Sara Spence (Neo) | CAN | 40.63 | | +1.830 |
| Saree Han (A2) | USA | 40.63 | PB | +1.830 |
| 19 | Altan-Ochir Zul (Neo) | MGL | 40.70 | NR | +1.900 |
| 20 | Gabrielle Sanson (A1) | CAN | 40.87 | | +2.070 |
| 21 | Allison Bates (Neo) | GBR | 41.19 | | +2.390 |
| 22 | Taylor White (Neo) | CAN | 41.23 | PB | +2.430 |
| 23 | Lindsey Kent (Neo) | CAN | 41.25 | | +2.450 |
| 24 | Bianca-Lorena Stănică (Neo) | ROU | 41.41 | SB | +2.610 |
| 25 | Hannah Clark (Senior) | CAN | 41.46 | PB | +2.660 |
| 26 | Mariah Richardson (Senior) | USA | 41.47 | | +2.670 |
| 27 | Connie Kapak (A2) | CAN | 41.63 | PB | +2.830 |
| Alexa Scott (B2) | CAN | 41.63 | | +2.830 |
| 29 | Yuna Lovell (B1) | CAN | 41.65 | | +2.850 |
| 30 | Sierra Johnson (A2) | CAN | 41.69 | | +2.890 |
| 31 | Blair Cruikshank (B2) | USA | 41.92 | | +3.120 |
| 32 | Sara Rehklau (Neo) | USA | 42.17 | | +3.370 |
| 33 | Olivia Moyse (A1) | CAN | 42.30 | SB | +3.500 |
| 34 | Danielle Dumouchel (A2) | CAN | 42.31 | | +3.510 |
| 35 | Andreea Haldan (A2) | ROU | 42.32 | | +3.520 |
| 36 | Anna Quinn (A1) | USA | 42.71 | | +3.910 |
| 37 | Sylvie Lloyd (Neo) | CAN | 43.50 | SB | +4.700 |
| 38 | Muriel Kennedy (Neo) | USA | 43.52 | PB | +4.720 |
| 39 | Kiersten Hagen (Neo) | CAN | 43.53 | | +4.730 |
| 40 | Rebecca Simmons (Senior) | USA | 43.62 | | +4.820 |
| 41 | Anna Petutschnigg (B1) | AUT | 43.79 | SB | +4.990 |
| 42 | Krissa Miller (M30) | USA | 43.84 | | +5.040 |
| 43 | Sydney-Yu Terpening (B2) | USA | 43.90 | | +5.100 |
| 44 | Jacquelyn Bernico (Neo) | USA | 43.93 | | +5.130 |
| 45 | Shruti Kotwal (Senior) | IND | 43.99 | | +5.190 |
| 46 | Martina Antifay (C2) | CAN | 44.05 | PB | +5.250 |
| 47 | Vanessa Coutu (B2) | CAN | 44.06 | | +5.260 |
| 48 | Meela Riben (C1) | CAN | 44.08 | SB | +5.280 |
| 49 | Lily Weidemann (A2) | CAN | 44.43 | | +5.630 |
| 50 | Jasmine Chase (Neo) | CAN | 44.54 | | +5.740 |
| 51 | Amanda Mitchell (B1) | CAN | 44.60 | | +5.800 |
| Molly Johnson (B2) | CAN | 44.60 | | +5.800 |
| 53 | Janelle Wiebe (A1) | CAN | 44.63 | | +5.830 |
| 54 | Zuzana Nedeljakova (B1) | CAN | 44.70 | | +5.900 |
| 55 | Annika Marshall (C2) | CAN | 44.75 | | +5.950 |
| 56 | Elliot Ganas (B1) | CAN | 44.83 | | +6.030 |
| 57 | Anna Bourgeois (A1) | CAN | 45.00 | | +6.200 |
| Grace Oh (C2) | USA | 45.00 | | +6.200 |
| 59 | Stefanie Spicer (A1) | CAN | 45.06 | | +6.260 |
| 60 | Kennedy Charles (B2) | CAN | 45.13 | | +6.330 |
| Ashley Hannah (A2) | CAN | 45.13 | | +6.330 |
| 62 | Brooke Braun (C2) | CAN | 45.15 | | +6.350 |
| 63 | Hannah Brunn (A2) | CAN | 45.23 | | +6.430 |
| 64 | Brianne Cipywnyk (B2) | CAN | 45.31 | | +6.510 |
| 65 | Chloe Beauchesne (B1) | CAN | 45.71 | | +6.910 |
| 66 | Ophelie Couture (B1) | CAN | 45.81 | PB | +7.010 |
| 67 | Melanie Pankow (C2) | USA | 45.90 | | +7.100 |
| 68 | Madeline Lange (B2) | USA | 46.03 | | +7.230 |
| 69 | Ceili Bracha (C2) | CAN | 46.17 | | +7.370 |
| 70 | Emma Javra (A1) | CAN | 46.37 | | +7.570 |
| 71 | Ilsa Melin Sandvig Shobe (A2) | USA | 46.50 | | +7.700 |
| 72 | Laura Hall (C2) | CAN | 46.64 | SB | +7.840 |
| 73 | Kali-Ann Friesen (B1) | CAN | 46.68 | SB | +7.880 |
| 74 | Yasmine Uden (A1) | CAN | 46.76 | | +7.960 |
| 75 | Zoe Lueders (C2) | CAN | 46.93 | | +8.130 |
| 76 | Jessica Telizyn (B2) | CAN | 47.18 | SB | +8.380 |
| 77 | Cassidy Peterson (A2) | CAN | 47.64 | | +8.840 |
| 78 | Emma Varden (A1) | CAN | 47.77 | | +8.970 |
| 79 | Shannon Dallaire (C2) | CAN | 48.38 | | +9.580 |
| 80 | Danica Grierson (C2) | CAN | 48.57 | SB | +9.770 |
| 81 | Alexis Havener (C2) | USA | 48.75 | | +9.950 |
| 82 | Gracie Reynoldson (D2) | CAN | 48.85 | SB | +10.050 |
| 83 | Samara Thew (Neo) | CAN | 49.00 | | +10.200 |
| 84 | Mara Jenkins (B1) | CAN | 49.15 | PB | +10.350 |
| 85 | Emma Roche (C2) | CAN | 49.23 | SB | +10.430 |
| 86 | Janaye Stribbell (C1) | CAN | 49.89 | | +11.090 |
| 87 | Kiera Bracha (D2) | CAN | 50.17 | | +11.370 |
| 88 | Mia Bantle (C2) | CAN | 50.57 | SB | +11.770 |
| 89 | Victoria Kalyniuk (C2) | CAN | 50.84 | | +12.040 |
| 90 | Elizabeth Carlisle (Neo) | CAN | 51.76 | | +12.960 |
| 91 | Emilee Stewart (B1) | CAN | 52.57 | PB | +13.770 |
| 92 | Peyton Stonehouse-Smith (B1) | CAN | 55.10 | | +16.300 |
| DNS | Ksenija Sadovskaja (Senior) | BLR | DNS | | - |
| DNS | Jenna Merth (Neo) | CAN | DNS | | - |
| DNS | Olivia Scott (Neo) | CAN | DNS | | - |
| DQ | Giorgia Birkeland (B1) | USA | DQ | | - |