| 1 | Alexa Scott (Senior) | CAN | 39.00 | | - |
| 2 | Maddison Pearman (Senior) | CAN | 39.19 | | +0.190 |
| 3 | Darby Beeson (Neo) | CAN | 39.85 | | +0.850 |
| 4 | Zhiyuan Wan (B1) | CHN | 39.86 | | +0.860 |
| 5 | Blair Cruikshank (Senior) | USA | 39.87 | | +0.870 |
| 6 | Tatiana de Vlieg (A2) | CAN | 39.94 | | +0.940 |
| 7 | Blanche Cardinal Tremblay (Neo) | CAN | 40.15 | | +1.150 |
| 8 | Katja Franzen (Senior) | GER | 40.73 | | +1.730 |
| 9 | Libby Williams (Neo) | USA | 41.00 | | +2.000 |
| 10 | Aoife Marshall (Neo) | CAN | 41.11 | | +2.110 |
| 11 | Myla Ireland (B1) | CAN | 41.18 | PB | +2.180 |
| 12 | Anna Quinn (Senior) | USA | 41.31 | | +2.310 |
| 13 | Zhixian.09 Wan (B1) | CHN | 41.41 | | +2.410 |
| 14 | Serena Dallaire (Neo) | CAN | 41.54 | SB | +2.540 |
| 15 | Ilsa Melin Sandvig Shobe (Senior) | USA | 41.58 | | +2.580 |
| 16 | Mia Tetrault (C2) | CAN | 42.52 | PB | +3.520 |
| 17 | Willa Dowse (B1) | CAN | 42.65 | | +3.650 |
| 18 | Ana Monroy (B2) | CAN | 42.87 | PB | +3.870 |
| 19 | Cassandra Currie (A2) | CAN | 43.03 | PB | +4.030 |
| 20 | Lena Veeman (C2) | CAN | 43.06 | PB | +4.060 |
| 21 | Daria Vogt (Neo) | CAN | 43.32 | | +4.320 |
| 22 | Mara Fuhrmann (A2) | GER | 43.43 | | +4.430 |
| 23 | Rebecca Currie (B1) | CAN | 43.62 | PB | +4.620 |
| 24 | Cynthia Jennings (M30) | USA | 43.72 | | +4.720 |
| 25 | Evyn Carr (B1) | CAN | 43.87 | SB | +4.870 |
| 26 | Lindsay Smart (Neo) | CAN | 43.96 | | +4.960 |
| Zosia Adelman (B1) | CAN | 43.96 | SB | +4.960 |
| 28 | Nastasja Radulovic (Neo) | USA | 44.38 | SB | +5.380 |
| 29 | Zitong Xu (D2) | CHN | 44.58 | | +5.580 |
| 30 | Hannah North (Neo) | CAN | 44.63 | | +5.630 |
| 31 | Gloria Han (C2) | CAN | 44.69 | PB | +5.690 |
| 32 | Nora Munro (B2) | CAN | 44.70 | | +5.700 |
| 33 | Kamaya Makowsky (B2) | CAN | 44.81 | SB | +5.810 |
| 34 | Genevieve Robinson (B2) | CAN | 44.82 | | +5.820 |
| 35 | Mollie Poulin (C2) | CAN | 44.86 | PB | +5.860 |
| 36 | Arya Jahns (B2) | CAN | 44.95 | | +5.950 |
| 37 | Ida Padbury (B1) | CAN | 45.28 | PB | +6.280 |
| 38 | Kate Folmer (B2) | CAN | 45.47 | PB | +6.470 |
| 39 | Megan Vansickle (B2) | CAN | 45.72 | PB | +6.720 |
| 40 | Tanessa Morris (Senior) | CAN | 46.16 | | +7.160 |
| 41 | Elliana Fickel (B1) | USA | 46.45 | | +7.450 |
| 42 | Tara Ashrafi (B1) | CAN | 46.52 | SB | +7.520 |
| 43 | Kayleigh Roberts (C2) | CAN | 46.62 | PB | +7.620 |
| 44 | Sophia Cairo Martinez (B2) | CAN | 46.66 | | +7.660 |
| 45 | Charlotte Perkins (B1) | CAN | 47.00 | PB | +8.000 |
| 46 | Jillian Reeves (Neo) | CAN | 47.18 | | +8.180 |
| 47 | Isabel Erasmus (Neo) | CAN | 47.44 | | +8.440 |
| 48 | Paulina Davydova (C2) | CAN | 47.49 | PB | +8.490 |
| Yi Lai (E2) | CHN | 47.49 | | +8.490 |
| 50 | Katharine Flemington (B1) | CAN | 47.77 | PB | +8.770 |
| 51 | Margaret Breedlove (A1) | CAN | 47.91 | | +8.910 |
| 52 | Amaranth Wang (C2) | CAN | 48.05 | PB | +9.050 |
| 53 | Meredith Armstrong (C2) | CAN | 48.91 | PB | +9.910 |
| 54 | Poppy Wheelhouse (C2) | CAN | 49.10 | | +10.100 |
| 55 | Ines Gonzalez De La Fuente (A1) | ESP | 49.20 | PB | +10.200 |
| 56 | Renata Kenzie (A1) | CAN | 50.79 | SB | +11.790 |
| 57 | Pareesa Jones (A2) | CAN | 51.84 | PB | +12.840 |
| 58 | Linna Zeibin (C2) | CAN | 56.21 | | +17.210 |
| 59 | Samaira Nemeth (B1) | CAN | 59.41 | | +20.410 |
| 60 | Jan Zurcher (M70) | USA | 59.84 | SB | +20.840 |
| DQ | Madison Fox (C2) | CAN | DQ | | - |