| 1 | Ying-Chu Chen (Senior) | TPE | 38.60 | SB | - |
| 2 | Heather Carruthers (Senior) | CAN | 38.76 | SB | +0.160 |
| 3 | Jenna Larter (Neo) | CAN | 38.93 | | +0.330 |
| 4 | Blair Cruikshank (Neo) | USA | 39.32 | SB | +0.720 |
| 5 | Alison Desmarais (Senior) | CAN | 39.51 | SB | +0.910 |
| 6 | Abigail Mccluskey (Senior) | CAN | 39.55 | SB | +0.950 |
| 7 | Anna Quinn (Senior) | USA | 40.25 | SB | +1.650 |
| 8 | Victoria Goplen (Neo) | CAN | 40.63 | PB | +2.030 |
| 9 | Sofia Bieber (A1) | CAN | 41.02 | | +2.420 |
| 10 | Anna Bourgeois (Senior) | CAN | 41.10 | | +2.500 |
| 11 | Caitlin Pelkey (Neo) | CAN | 41.73 | SB | +3.130 |
| 12 | Rachel Mallard (Neo) | CAN | 42.05 | PB | +3.450 |
| 13 | Ilsa Melin Sandvig Shobe (Senior) | USA | 42.45 | SB | +3.850 |
| 14 | Hayleigh Bell (Senior) | CAN | 42.64 | | +4.040 |
| 15 | Blanche Cardinal Tremblay (A1) | CAN | 42.69 | | +4.090 |
| 16 | Molly Johnson (Neo) | CAN | 42.74 | | +4.140 |
| 17 | Sydney-Yu Terpening (Neo) | USA | 43.05 | | +4.450 |
| 18 | Sophia Dvorak (B2) | CAN | 43.43 | SB | +4.830 |
| 19 | Keeley Whitcomb (B1) | USA | 44.15 | SB | +5.550 |
| 20 | Hannah North (Neo) | CAN | 44.58 | | +5.980 |
| 21 | Elise Stave (B1) | USA | 44.70 | SB | +6.100 |
| 22 | Kamaya Makowsky (C2) | CAN | 44.80 | PB | +6.200 |
| 23 | Sophia Major (B1) | CAN | 45.45 | SB | +6.850 |
| 24 | Cheyenne Key (A1) | CAN | 45.46 | PB | +6.860 |
| 25 | Arya Jahns (C2) | CAN | 45.72 | PB | +7.120 |
| 26 | Isabel Erasmus (A1) | CAN | 45.73 | SB | +7.130 |
| 27 | Elena Stave (C1) | USA | 45.86 | SB | +7.260 |
| 28 | Sophia Cairo Martinez (C2) | CAN | 46.24 | PB | +7.640 |
| 29 | Maya Kemp (A1) | CAN | 46.34 | | +7.740 |
| 30 | Talia Halperin (B1) | CAN | 47.12 | PB | +8.520 |
| 31 | Heidi Beck (M30) | CAN | 47.73 | | +9.130 |
| 32 | Julianne Lazzari (B1) | CAN | 48.16 | | +9.560 |
| 33 | Genevieve Robinson (C2) | CAN | 48.60 | SB | +10.000 |
| 34 | Kim Reid (M50) | CAN | 49.46 | SB | +10.860 |
| 35 | Mya Onos (A1) | CAN | 49.59 | PB | +10.990 |
| 36 | Diane Silzer (M60) | CAN | 49.89 | SB | +11.290 |
| 37 | Cara Byrne (C2) | CAN | 50.87 | | +12.270 |
| 38 | Xingyan Han (C2) | CAN | 51.36 | SB | +12.760 |
| 39 | Karen Doolan (M65) | CAN | 1:02.75 | SB | +24.150 |