| 1 | Carolina Hiller-Donnelly (Senior) | CAN | 39.56 | | - |
| 2 | Abigail Mccluskey (Senior) | CAN | 39.81 | | +0.250 |
| 3 | Kali Christ (Senior) | CAN | 40.05 | | +0.490 |
| 4 | Blair Cruikshank (Neo) | USA | 40.30 | | +0.740 |
| 5 | Hayleigh Bell (Senior) | CAN | 41.04 | SB | +1.480 |
| 6 | Victoria Goplen (Neo) | CAN | 41.38 | | +1.820 |
| 7 | Sofia Bieber (B1) | CAN | 41.72 | | +2.160 |
| 8 | Anna Bourgeois (Neo) | CAN | 41.75 | | +2.190 |
| 9 | Laura Hall (A2) | CAN | 41.99 | | +2.430 |
| 10 | Caitlin Pelkey (Neo) | CAN | 42.45 | | +2.890 |
| 11 | Kiersten Hagen (Senior) | CAN | 42.51 | | +2.950 |
| Abbey Young (Neo) | CAN | 42.51 | PB | +2.950 |
| 13 | Ellie Fuchs (Neo) | USA | 42.54 | | +2.980 |
| 14 | Molly Johnson (Neo) | CAN | 42.56 | | +3.000 |
| 15 | Rachel Freitag (Neo) | CAN | 42.57 | PB | +3.010 |
| 16 | Skylar van Horne (C2) | CAN | 42.61 | | +3.050 |
| 17 | Sydney-Yu Terpening (Neo) | USA | 42.85 | | +3.290 |
| 18 | Claire Mallard (Neo) | CAN | 42.87 | | +3.310 |
| 19 | Sylvie Lloyd (Senior) | CAN | 42.88 | | +3.320 |
| 20 | Zoe Lueders (A2) | CAN | 42.93 | | +3.370 |
| 21 | Jia Griffiths (A1) | USA | 43.13 | SB | +3.570 |
| 22 | Diana-Andreea Nastasache (Neo) | ROU | 43.14 | | +3.580 |
| 23 | Meela Riben (A1) | CAN | 43.16 | | +3.600 |
| 24 | Ilsa Melin Sandvig Shobe (Neo) | USA | 43.23 | | +3.670 |
| 25 | Robyn Salie (A1) | CAN | 43.26 | | +3.700 |
| 26 | Cynthia Jennings (Senior) | USA | 43.38 | | +3.820 |
| 27 | Nele Charline Neumann (B1) | GER | 43.54 | | +3.980 |
| 28 | Emma Langstrom (Neo) | CAN | 43.73 | | +4.170 |
| 29 | Alexa Annecca (Neo) | CAN | 44.00 | | +4.440 |
| 30 | Ella Cloutier (B1) | CAN | 44.17 | | +4.610 |
| 31 | Kali-Ann Friesen (Neo) | CAN | 44.19 | | +4.630 |
| 32 | Brooke Braun (A2) | CAN | 44.32 | | +4.760 |
| 33 | Serena Dallaire (B1) | CAN | 44.65 | | +5.090 |
| 34 | Lindsay Smart (B1) | CAN | 44.83 | | +5.270 |
| 35 | Sidney Bennie (B2) | CAN | 44.87 | PB | +5.310 |
| 36 | Maya Kemp (B1) | CAN | 44.95 | PB | +5.390 |
| 37 | Jelena Manina (B1) | BLR | 45.49 | PB | +5.930 |
| 38 | Camille Alford (A1) | CAN | 45.90 | | +6.340 |
| 39 | Hannah North (A1) | CAN | 46.50 | | +6.940 |
| 40 | Antonia Villa (B2) | CAN | 46.82 | | +7.260 |
| 41 | Rachel Spridzans (B2) | CAN | 47.08 | | +7.520 |
| 42 | Gracie Reynoldson (B2) | CAN | 47.65 | | +8.090 |
| 43 | Cheyenne Key (B1) | CAN | 47.80 | SB | +8.240 |
| 44 | Elizabeth.05 Smith (B1) | CAN | 48.42 | | +8.860 |
| 45 | Heidi Beck (Senior) | CAN | 48.72 | SB | +9.160 |
| 46 | Calla Haaheim (C2) | CAN | 49.73 | | +10.170 |
| 47 | Grier Kellough (C1) | CAN | 50.29 | PB | +10.730 |
| 48 | Denise Wesseling (C2) | CAN | 50.48 | | +10.920 |
| 49 | Julia Smith (B1) | CAN | 50.60 | PB | +11.040 |
| 50 | Talia Halperin (C1) | CAN | 51.59 | | +12.030 |
| 51 | Molly Morris (A2) | CAN | 52.41 | | +12.850 |
| 52 | Margaret Breedlove (C1) | CAN | 55.98 | | +16.420 |
| DNF | Sophia Dvorak (C2) | CAN | DNF | | - |