| 1 | Lucy Bollert (D2) | GER | 44.86 | SB | - |
| 2 | Emma Charlotte Wolf (C1) | GER | 45.57 | | +0.710 |
| 3 | Jordine Emma Altkrüger (D2) | GER | 45.90 | PB | +1.040 |
| 4 | Luna-Luisa Powik (D2) | GER | 46.17 | | +1.310 |
| 5 | Laura Altenhof (B1) | GER | 46.36 | | +1.500 |
| 6 | Alexandra Sauer (D1) | GER | 47.06 | SB | +2.200 |
| 7 | Clarissa Pagano (D2) | GER | 47.16 | | +2.300 |
| 8 | Paula König (D2) | GER | 47.33 | SB | +2.470 |
| 9 | Charlotte Pfau (D2) | GER | 48.61 | | +3.750 |
| 10 | Luisa Jarmatz (D1) | GER | 49.16 | | +4.300 |
| 11 | Emely Schürmann (D1) | GER | 49.62 | SB | +4.760 |
| 12 | Neele Göpelt (E2) | GER | 49.76 | | +4.900 |
| 13 | Marieke Diefenbach (D1) | GER | 51.04 | | +6.180 |
| 14 | Anik Podufall (D1) | GER | 51.69 | | +6.830 |
| 15 | Anna Diatchenko (E2) | GER | 52.32 | | +7.460 |
| 16 | Lina Kaiser (E2) | GER | 52.52 | SB | +7.660 |
| 17 | Kaily Wuttig (D2) | GER | 53.31 | | +8.450 |
| 18 | Paulina Klein (D2) | GER | 53.38 | PB | +8.520 |
| 19 | Lucie Golde (E2) | GER | 53.42 | SB | +8.560 |
| 20 | Vanessa Neumann (D2) | GER | 53.74 | | +8.880 |
| 21 | Anna Weidelt (D1) | GER | 54.04 | | +9.180 |
| 22 | Vivienne Wagenknecht (D1) | GER | 54.58 | PB | +9.720 |
| 23 | Julienne Rogge (D1) | GER | 55.11 | | +10.250 |
| 24 | Isa de Souza Ferraz Höllein (E2) | GER | 55.27 | | +10.410 |
| 25 | Leni Hoffmann (F2) | GER | 55.49 | SB | +10.630 |
| 26 | Florentine Blümel (E2) | GER | 55.86 | | +11.000 |
| 27 | Nea Malin Schöbel (D1) | GER | 55.94 | | +11.080 |
| 28 | Helena Unterdörfel (F2) | GER | 59.19 | SB | +14.330 |
| 29 | Helena Hoffmann (E1) | GER | 59.95 | SB | +15.090 |
| 30 | Lyann Lukas (E1) | GER | 1:01.60 | SB | +16.740 |
| 31 | Thea Luisa Schmidt (E2) | GER | 1:01.70 | PB | +16.840 |
| 32 | Marie Köbernick (E1) | GER | 1:02.88 | PB | +18.020 |
| 33 | Frederike Graf (E2) | GER | 1:03.49 | PB | +18.630 |
| 34 | Enrika Franziska Sczepansky (E2) | GER | 1:04.01 | SB | +19.150 |
| 35 | Ellen Martin (E1) | GER | 1:04.42 | PB | +19.560 |
| 36 | Annika Vicenti (E2) | GER | 1:05.88 | | +21.020 |
| 37 | Annegreth Diefenbach (E1) | GER | 1:07.81 | | +22.950 |
| 38 | Katja Postolaki (E1) | GER | 1:09.29 | SB | +24.430 |
| 39 | Jonna Linea Podufall (E1) | GER | 1:09.98 | SB | +25.120 |
| 40 | Gianna Sophie Sarnoch (D1) | GER | 1:10.60 | | +25.740 |
| 41 | Franka Schniesko (E2) | GER | 1:10.82 | | +25.960 |
| 42 | Novalie Bergunde (E2) | GER | 1:13.11 | PB | +28.250 |
| 43 | Julia Walter (E2) | GER | 1:18.91 | SB | +34.050 |