| 1 | Miki Aro (Neo) | JPN | 39.04 | | - |
| 2 | Maria Victoria Rodriguez (Senior) | ARG | 39.31 | SB | +0.270 |
| 3 | Fumina Ishioka (A1) | JPN | 39.51 | PB | +0.470 |
| 4 | Sakura Mito (Neo) | JPN | 40.05 | PB | +1.010 |
| 5 | Blair Cruikshank (Senior) | USA | 40.10 | | +1.060 |
| 6 | Victoria Goplen (Neo) | CAN | 40.15 | | +1.110 |
| Towako Nagasaki (Senior) | JPN | 40.15 | | +1.110 |
| 8 | Piper Yde (Neo) | USA | 40.33 | PB | +1.290 |
| 9 | Anna Quinn (Senior) | USA | 40.71 | | +1.670 |
| 10 | Libby Williams (Neo) | USA | 41.11 | | +2.070 |
| 11 | Marley Soldan (A1) | USA | 41.40 | | +2.360 |
| 12 | Maya Lueders (A2) | CAN | 41.44 | | +2.400 |
| 13 | Amy Acosta (Neo) | ECU | 41.51 | NR | +2.470 |
| 14 | Ilsa Melin Sandvig Shobe (Senior) | USA | 41.56 | | +2.520 |
| 15 | Jenni Kukkonen (M35) | FIN | 41.57 | NRM | +2.530 |
| 16 | Giorgia Birkeland (Neo) | USA | 41.67 | SB | +2.630 |
| 17 | Karen Lizeth Siatama Valencia (A2) | COL | 42.31 | | +3.270 |
| 18 | Elise Stave (B2) | USA | 42.35 | PB | +3.310 |
| 19 | Natalia Valencia (Senior) | COL | 42.49 | | +3.450 |
| 20 | Keeley Whitcomb (B2) | USA | 42.78 | | +3.740 |
| 21 | Larissa Paes (M30) | BRA | 42.80 | NRM | +3.760 |
| 22 | Elena Stave (C2) | USA | 43.20 | | +4.160 |
| 23 | Cynthia Jennings (Senior) | USA | 43.36 | SB | +4.320 |
| 24 | Evie Forrer (B1) | USA | 43.71 | | +4.670 |
| 25 | Mary Brophy (M50) | USA | 44.13 | | +5.090 |
| 26 | Ella Teeples (B1) | USA | 44.45 | | +5.410 |
| 27 | Alejandra Huertas (B2) | COL | 44.54 | | +5.500 |
| 28 | Aileen Oh (C1) | USA | 45.64 | | +6.600 |
| 29 | Audrey Han (B2) | USA | 46.49 | | +7.450 |
| 30 | Shruti Kotwal (M30) | IND | 46.93 | | +7.890 |
| 31 | Kassidy González Mcglauchlen (C2) | USA | 48.13 | PB | +9.090 |
| DQ | Hina Onodera (Neo) | JPN | DQ | | - |