| 1 | Janine Smit (Senior) | NED | 38.91 | | - |
| Floor van den Brandt (Senior) | NED | 38.91 | | - |
| 3 | Bo van der Werff (Senior) | NED | 39.26 | | +0.350 |
| 4 | Letitia de Jong (Senior) | NED | 39.29 | | +0.380 |
| 5 | Sanneke de Neeling (Neo) | NED | 39.36 | | +0.450 |
| 6 | Ireen Wüst (Senior) | NED | 39.37 | | +0.460 |
| 7 | Jutta Leerdam (A1) | NED | 39.75 | | +0.840 |
| 8 | Antoinette de Jong (Neo) | NED | 39.79 | | +0.880 |
| 9 | Moniek Klijnstra (Senior) | NED | 39.98 | | +1.070 |
| 10 | Annouk van der Weijden (Senior) | NED | 40.03 | SB | +1.120 |
| 11 | Tessa Boogaard (Neo) | NED | 40.06 | | +1.150 |
| 12 | Dione Voskamp (Neo) | NED | 40.21 | | +1.300 |
| 13 | Leeyen Harteveld (Neo) | NED | 40.24 | | +1.330 |
| 14 | Linda de Vries (Senior) | NED | 40.37 | | +1.460 |
| 15 | Jorien Voorhuis (Senior) | NED | 41.11 | | +2.200 |
| 16 | Esther Kiel (Neo) | NED | 41.29 | | +2.380 |