| 1 | Demi Vollering (Neo) | NED | 44.63 | | - |
| 2 | Caithlyn Tschi (A2) | NED | 45.49 | SB | +0.860 |
| 3 | Fenna Bross (B1) | NED | 46.34 | | +1.710 |
| 4 | Esther Droogers (A2) | NED | 46.37 | | +1.740 |
| 5 | Nena Vollering (C2) | NED | 46.68 | | +2.050 |
| 6 | Viviënne de Jong (B2) | NED | 46.79 | SB | +2.160 |
| 7 | Liesbeth Veen-Milatz (Senior) | NED | 46.94 | | +2.310 |
| 8 | Stacey Lansbergen (Neo) | NED | 47.06 | SB | +2.430 |
| 9 | Patou de Haas (C1) | NED | 47.79 | | +3.160 |
| 10 | Nola Sloot (B1) | NED | 47.89 | | +3.260 |
| 11 | Silvia Ammerlaan (M45) | NED | 48.16 | PB | +3.530 |
| 12 | Demi Ammerlaan (A1) | NED | 48.18 | | +3.550 |
| 13 | Cheyenne van der Breggen (B1) | NED | 48.67 | SB | +4.040 |
| 14 | Frederieke Jansen (Neo) | NED | 48.68 | | +4.050 |
| 15 | Mirthe van der Beek (A1) | NED | 48.78 | | +4.150 |
| 16 | Heleen Docter (A2) | NED | 48.85 | | +4.220 |
| 17 | Inge Bos (Neo) | NED | 49.21 | SB | +4.580 |
| 18 | Bodine Vollering (D2) | NED | 49.66 | | +5.030 |
| 19 | Marianne de Neeling (M55) | NED | 50.20 | | +5.570 |
| 20 | Emma Noz (D1) | NED | 50.55 | | +5.920 |
| 21 | Julia van den Berg (C2) | NED | 52.30 | | +7.670 |
| 22 | Renee van der Ham (C1) | NED | 52.47 | SB | +7.840 |
| 23 | Annemaj Scholte (A1) | NED | 52.64 | | +8.010 |
| 24 | Marlou van Winden (C1) | NED | 54.09 | | +9.460 |
| 25 | Germaine van Kesteren (B2) | NED | 54.25 | SB | +9.620 |
| 26 | Dianne Vos (C1) | NED | 54.54 | SB | +9.910 |
| 27 | Jenny Zuidgeest (Senior) | NED | 55.75 | SB | +11.120 |
| 28 | Floor ter Wal (M40) | NED | 55.95 | SB | +11.320 |
| 29 | Janique Scholte (C1) | NED | 1:03.16 | | +18.530 |
| 30 | Jacqueline Bos (M55) | NED | 1:04.32 | SB | +19.690 |
| Anjellina Tschi-Reen (M45) | NED | 1:04.32 | SB | +19.690 |
| 32 | Anne van Oosterhout (A2) | NED | 1:06.27 | SB | +21.640 |
| 33 | Janneke de Ruiter (M40) | NED | 1:06.79 | PB | +22.160 |
| 34 | Lin Mae Tschi (D2) | NED | 1:10.65 | | +26.020 |
| 35 | Nel Kleijweg (M50) | NED | 1:11.51 | SB | +26.880 |
| 36 | Emma van Lienden (D2) | NED | 1:17.30 | SB | +32.670 |
| DNS | Janine Noordam (A2) | NED | DNS | | - |