| 1 | Sarina van Dijk (Neo) | NED | 45.51 | | - |
| 2 | Chiara Adams (Senior) | NED | 45.67 | SB | +0.160 |
| 3 | Bo Mengerink (A1) | NED | 46.55 | | +1.040 |
| 4 | Bryndis van Schijndel (A1) | NED | 49.03 | | +3.520 |
| 5 | Meike Wagemakers (Neo) | NED | 49.61 | | +4.100 |
| 6 | Yara van Klaveren (C2) | NED | 49.97 | | +4.460 |
| 7 | Ilse Vanlaerhoven (M35) | NED | 53.02 | SB | +7.510 |
| 8 | Lieve de Roon (M35) | NED | 54.16 | SB | +8.650 |
| 9 | Maartje Mulders (Senior) | NED | 54.46 | SB | +8.950 |
| 10 | Gerita Smallegoor (M50) | NED | 54.51 | | +9.000 |
| 11 | Anouk de Jong (Senior) | NED | 54.75 | | +9.240 |
| 12 | Evelien Vissers Steneker (M35) | NED | 56.36 | SB | +10.850 |
| 13 | Patty van Hoorn (M50) | NED | 56.90 | | +11.390 |
| 14 | Brechtje van der Zwet - de Roon (M40) | NED | 58.01 | SB | +12.500 |
| 15 | Merel Kramer (D2) | NED | 58.95 | | +13.440 |
| 16 | Carlijn Thijssen (M30) | NED | 59.52 | SB | +14.010 |
| 17 | Ethel Pennings-Graat (M50) | NED | 1:05.49 | | +19.980 |
| 18 | Dalila Sayd (n/a) | NED | 1:05.87 | PB | +20.360 |
| 19 | Amy Biemans (D2) | NED | 1:08.41 | SB | +22.900 |
| 20 | Thera Habben-Jansen (M50) | NED | 1:08.48 | | +22.970 |
| 21 | Vienna van Dongen (E2) | NED | 1:15.42 | PB | +29.910 |
| 22 | Romy van Zuijlen (Neo) | NED | 1:25.04 | PB | +39.530 |