| 1 | Brittany Bowe (Senior) | USA | 38.22 | SB | - |
| 2 | Erin Jackson (Senior) | USA | 38.56 | SB | +0.340 |
| 3 | McKenzie Browne (Senior) | USA | 38.90 | SB | +0.680 |
| 4 | Chrysta Rands-Evans (Senior) | USA | 39.22 | SB | +1.000 |
| 5 | Mia Manganello (Senior) | USA | 40.11 | SB | +1.890 |
| 6 | Libby Williams (Neo) | USA | 41.38 | SB | +3.160 |
| 7 | Natalia Valencia (Senior) | COL | 42.17 | SB | +3.950 |
| 8 | Emma de Bock (B1) | USA | 42.25 | SB | +4.030 |
| 9 | Rebecca Simmons (M35) | USA | 43.23 | SB | +5.010 |
| 10 | Larissa Paes (Senior) | BRA | 43.24 | SB | +5.020 |
| 11 | Shruti Kotwal (M30) | IND | 43.26 | SB | +5.040 |
| 12 | Natascha Lindenskov (Neo) | DEN | 44.32 | SB | +6.100 |
| 13 | Cynthia Jennings (Senior) | USA | 44.91 | SB | +6.690 |
| 14 | Amy Acosta (n/a) | ECU | 46.39 | NR | +8.170 |
| 15 | Dasha Epperson (n/a) | USA | 52.67 | SB | +14.450 |